Artículo
Identification of cattle carrying alleles associated with resistance and susceptibility to the Bovine Leukemia Virus progression by real-time PCR
Forletti, Agustina
; Juliarena, Marcela Alicia
; Ceriani, Maria Carolina
; Amadio, Ariel Fernando
; Esteban, Eduardo Nestor; Gutiérrez, Silvina Elena
Fecha de publicación:
08/2013
Editorial:
Elsevier
Revista:
Research In Veterinary Science
ISSN:
0034-5288
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Abstract Previous studies have shown a significant association between polymorphisms of the BoLA DRB3 gene and Bovine Leukemia Virus (BLV) infection profile. The presence of allele ∗1501 has been associated with high proviral load in peripheral blood while allele ∗0902 has been associated with low proviral load. The purpose of this study was to develop allele-specific real-time PCRs to identify cattle carrying alleles associated with resistance (BoLA DRB3∗0902) or susceptibility (BoLA DRB3∗1501) to the BLV progression. Specific primers were designed and differential amplification was carried out by real-time PCR and monitored by SYBR® Green dye in DNA samples from peripheral blood. Conditions were also adjusted for traditional PCR amplification (end point amplification). These methods are rapid, simple and suitable for high throughput screening, and could aid in marker-assisted selection of BLV-resistant and susceptible cattle.
Palabras clave:
Bola Drb3
,
Bovine Leukemia Virus
,
Resistance
,
Susceptibility
,
Pcr
,
Rea-Time Pcr
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CCT - SANTA FE)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SANTA FE
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SANTA FE
Articulos(CIVETAN)
Articulos de CENTRO DE INVESTIGACION VETERINARIA DE TANDIL
Articulos de CENTRO DE INVESTIGACION VETERINARIA DE TANDIL
Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Articulos de SEDE CENTRAL
Citación
Forletti, Agustina; Juliarena, Marcela Alicia; Ceriani, Maria Carolina; Amadio, Ariel Fernando; Esteban, Eduardo Nestor; et al.; Identification of cattle carrying alleles associated with resistance and susceptibility to the Bovine Leukemia Virus progression by real-time PCR; Elsevier; Research In Veterinary Science; 95; 3; 8-2013; 991–995
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