Artículo
Differential expression of low density lipoprotein receptor–related protein 1 (LRP-1) and matrix metalloproteinase-9 (MMP-9) in prostate gland: From normal to malignant lesions
Gilardoni, Mónica Beatriz; Remedi, Maria Monica
; Oviedo, Mabel; Dellavedova, Tristán; Sarría, Juan P.; Racca, Laura; Dominguez, Mariana; Pellizas, Claudia Gabriela
; Donadio, Ana Carolina
Fecha de publicación:
01/2017
Editorial:
Elsevier Gmbh
Revista:
Pathology - Research And Practice
ISSN:
0344-0338
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Background Metalloproteinases (MMPs) are relevant modulators of inflammation, tumor microenvironment, cancer invasion and metastasis. They can be regulated by the Low density lipoprotein Receptor?related Protein 1 (LRP-1), a receptor reported to mediate the clearance of lipoproteins, extracellular matrix (ECM) macromolecules and proteinases. The aim of this study was to evaluate the expression of LRP-1, MMP-2 and MMP-9 across various grades of prostatic diseases as benign prostatic hyperplasia (BPH), BPH plus prostatitis (BPH + P), high grade prostatic intraepithelial neoplasia (HGPIN) and prostate cancer (PCa). Methods LRP-1 was analyzed using immunohistochemistry and MMPs proteolytic activity by zymography in prostate tissues with different prostatic diseases. Results LRP-1 was detected in epithelial cells in BPH (16/18), BPH + P (21/21) and HGPIN (6/6), with a staining intensity of 1+, 1+/2+ and 3+, respectively. In PCa, LRP-1 was absent in 19/27 samples while a low expression was observed in 8/27 biopsies. MMP-9 activity was higher and statistically significant in PCa than in BPH (p ≤ 0.01). Conclusion Considering that LRP-1, by mediating the clearance of MMPs, is involved in the regulation of ECM remodeling and cell migration, we conclude that a decreased expression of LRP-1 could be involved with the increasing activity of MMPs shown in cancers.
Palabras clave:
Cancer
,
Immunohistochemistry
,
Lrp-1
,
Mmp-9
,
Prostate
,
Zymography
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Identificadores
Colecciones
Articulos(CIBICI)
Articulos de CENTRO DE INV.EN BIOQUI.CLINICA E INMUNOLOGIA
Articulos de CENTRO DE INV.EN BIOQUI.CLINICA E INMUNOLOGIA
Citación
Gilardoni, Mónica Beatriz; Remedi, Maria Monica; Oviedo, Mabel; Dellavedova, Tristán; Sarría, Juan P.; et al.; Differential expression of low density lipoprotein receptor–related protein 1 (LRP-1) and matrix metalloproteinase-9 (MMP-9) in prostate gland: From normal to malignant lesions; Elsevier Gmbh; Pathology - Research And Practice; 213; 1; 1-2017; 66-71
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