Mostrar el registro sencillo del ítem
dc.contributor.author
Racca, Ana Cristina
dc.contributor.author
Ridano, Magali Evelin
dc.contributor.author
Bandeira, C. L.
dc.contributor.author
Avvad Portari, E.
dc.contributor.author
Genti de Raimondi, Susana
dc.contributor.author
Graham, C. H.
dc.contributor.author
Panzetta-Dutari, Graciela Maria del Valle
dc.date.available
2018-05-31T14:19:03Z
dc.date.issued
2016-09
dc.identifier.citation
Racca, Ana Cristina; Ridano, Magali Evelin; Bandeira, C. L.; Avvad Portari, E.; Genti de Raimondi, Susana; et al.; Low oxygen tension induces Krüppel-Like Factor 6 expression in trophoblast cells; W B Saunders Co Ltd; Placenta; 45; 9-2016; 50-57
dc.identifier.issn
0143-4004
dc.identifier.uri
http://hdl.handle.net/11336/46773
dc.description.abstract
The transcription factor Krüppel-Like Factor 6 (KLF6) has important roles in cell differentiation, angiogenesis, apoptosis, and proliferation. Furthermore, there is evidence that KLF6 is required for proper placental development. While oxygen is a critical mediator of trophoblast differentiation and function, the involvement of oxygen in the regulation of KLF6 expression remains unexplored. In the present study we examined the expression of KLF6 in placental tissue from uncomplicated and preeclamptic pregnancies, the latter often characterized by an inadequately perfused placenta. We also determined the effect of hypoxia and the involvement of Hypoxia-Inducible Factor 1α (HIF-1α) on the expression of KLF6 in cultured trophoblast cells and placental tissues. Results revealed that villous, interstitial and endovascular extravillous cytotrophoblasts from placentas from normal and preeclamptic pregnancies express KLF6. In addition, KLF6 immunoreactivity was higher in the placental bed of preeclamptic pregnancies than in those of uncomplicated pregnancies. We demonstrated that hypoxia induced an early and transient increase in KLF6 protein levels in HTR8/SVneo extravillous cytotrophoblast cells and in placental explants. Reoxygenation returned KLF6 protein to basal levels. Moreover, hypoxia-induced up-regulation of KLF6 expression was dependent on HIF-1α as revealed by siRNA knockdown in HTR8/SVneo cells. These results indicate that KLF6 may mediate some of the effects of hypoxia in placental development. The regulation of KLF6 protein levels by oxygen has significant implications for understanding its putative role in diseases affected by tissue hypoxia.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
W B Saunders Co Ltd
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
HYPOXIA
dc.subject
HYPOXIA INDUCIBLE FACTOR-1Α
dc.subject
KRÜPPEL-LIKE FACTOR 6
dc.subject
PLACENTA
dc.subject
TROPHOBLAST
dc.subject.classification
Otras Ciencias Biológicas
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Low oxygen tension induces Krüppel-Like Factor 6 expression in trophoblast cells
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-05-22T21:49:09Z
dc.journal.volume
45
dc.journal.pagination
50-57
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Racca, Ana Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
dc.description.fil
Fil: Ridano, Magali Evelin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
dc.description.fil
Fil: Bandeira, C. L.. Universidade de Sao Paulo; Brasil
dc.description.fil
Fil: Avvad Portari, E.. Universidade Federal do Rio de Janeiro; Brasil
dc.description.fil
Fil: Genti de Raimondi, Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
dc.description.fil
Fil: Graham, C. H.. Queens University; Canadá
dc.description.fil
Fil: Panzetta-Dutari, Graciela Maria del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
dc.journal.title
Placenta
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0143400416303885
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.placenta.2016.07.006
Archivos asociados