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dc.contributor.author
Alvarez, Gabriel Martín
dc.contributor.author
Morado, Sergio Adrián
dc.contributor.author
Soto, Mariela Patricia
dc.contributor.author
Dalvit, Gabriel Carlos
dc.contributor.author
Cetica, Pablo Daniel
dc.date.available
2018-05-30T18:33:12Z
dc.date.issued
2015-04
dc.identifier.citation
Alvarez, Gabriel Martín; Morado, Sergio Adrián; Soto, Mariela Patricia; Dalvit, Gabriel Carlos; Cetica, Pablo Daniel; The Control of Reactive Oxygen Species Influences Porcine Oocyte In Vitro Maturation; Wiley Blackwell Publishing, Inc; Reproduction in Domestic Animals; 50; 2; 4-2015; 200-205
dc.identifier.issn
0936-6768
dc.identifier.uri
http://hdl.handle.net/11336/46695
dc.description.abstract
The aim of this study was to examine the effect of varying intracellular reactive oxygen species (ROS) levels during oocyte in vitro maturation with enzymatic ROS production systems (xanthine + xanthine oxidase or xanthine + xanthine oxidase + catalase), scavenger systems (catalase or superoxide dismutase + catalase) or cysteine on porcine oocyte maturation. Oocyte ROS levels showed an increase when H2O2 or O2∙(-) production systems were added to the culture medium (p < 0.05). On the other hand, the presence of ROS scavengers in the maturation medium did not modify oocyte ROS levels compared with the control after 48 h of maturation, but the addition of cysteine induced a decrease in oocyte ROS levels (p < 0.05). The ROS production systems used in this work did not modified the percentage of oocyte nuclear maturation, but increased the decondensation of sperm head (p < 0.05) and decreased the pronuclear formation (p < 0.05). In turn, the addition of O2∙(-) and H2O2 scavenging systems during in vitro maturation did not modify the percentage of oocytes reaching metaphase II nor the oocytes with decondensed sperm head or pronuclei after fertilization. However, both parameters increased in the presence of cysteine (p < 0.05). The exogenous generation of O2∙(-) and H2O2 during oocyte in vitro maturation would not affect nuclear maturation or later sperm penetration, but most of the spermatozoa cannot progress to form the pronuclei after fusion with the oocyte. The decrease in endogenous ROS levels by the addition of cysteine would improve pronuclear formation after sperm penetration.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Reactive Oxygen Species
dc.subject
In Vitro Maturation
dc.subject
Oocytes
dc.subject
Porcine
dc.subject.classification
Ciencias Veterinarias
dc.subject.classification
Ciencias Veterinarias
dc.subject.classification
CIENCIAS AGRÍCOLAS
dc.title
The Control of Reactive Oxygen Species Influences Porcine Oocyte In Vitro Maturation
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-05-23T16:34:48Z
dc.journal.volume
50
dc.journal.number
2
dc.journal.pagination
200-205
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Alvarez, Gabriel Martín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina
dc.description.fil
Fil: Morado, Sergio Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina
dc.description.fil
Fil: Soto, Mariela Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina
dc.description.fil
Fil: Dalvit, Gabriel Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina
dc.description.fil
Fil: Cetica, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina
dc.journal.title
Reproduction in Domestic Animals
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1111/rda.12469
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/rda.12469
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