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dc.contributor.author
Pascual, Ana Clara
dc.contributor.author
Gaveglio, Virginia Lucía
dc.contributor.author
Giusto, Norma Maria
dc.contributor.author
Pasquaré, Susana Juana
dc.date.available
2016-03-07T19:39:51Z
dc.date.issued
2013-03
dc.identifier.citation
Pascual, Ana Clara; Gaveglio, Virginia Lucía; Giusto, Norma Maria; Pasquaré, Susana Juana; Aging Modifies the Enzymatic Activities Involved in 2-Arachidonoylglycerol Metabolism; Wiley; Biofactors; 39; 2; 3-2013; 209-220
dc.identifier.issn
0951-6433
dc.identifier.uri
http://hdl.handle.net/11336/4666
dc.description.abstract
One of the principal monoacylglycerol (MAG) species in animal tissues is 2-arachidonoylglycerol (2-AG), and the diacylglycerol lipase (DAGL) pathway is the most important 2-AG biosynthetic pathway proposed to date. Lysophosphatidate phosphatase (LPAase) activity is part of another 2-AG-forming pathway in which monoacylglycerol lipase (MAGL) is the major degrading enzyme. The purpose of this study was to analyze the manner in which DAGL, LPAase, and MAGL enzymes are modified in the central nervous system (CNS) during aging. To this end, diacylglycerols (DAGs) and MAGs of different composition were used as substrates of DAGL and MAGL, respectively. All enzymatic activities were evaluated in membrane and soluble fractions as well as in synaptic terminals from the cerebral cortex (CC) of adult and aged rats. Results related to 2-AG metabolism show that aging: (a) decreases DAGL-alfa expression in the membrane fraction whereas in synaptosomes it increases DAGL-beta and decreases MAGL expression; (b) decreases LPAase activity in both membrane and soluble fractions; (c) decreases DAGL and stimulates LPAase activities in CC synaptic terminals; (d) stimulates membrane-associated MAGL-coupled DAGL activity; and (e) stimulates MAGL activity in CC synaptosomes. Our results also reveal that during aging the net balance between the enzymatic activities involved in 2-AG synthesis and breakdown is low availability of 2-AG in CC membrane fractions and synaptic terminals. Taken together, our results lead us to conclude that these enzymes play crucial roles in the regulation of 2-AG tissue levels during aging.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
2-Arachidonoylglycerol
dc.subject
Cerebral Cortex
dc.subject
Lysophosphatidic Acid
dc.subject
Synaptosomes
dc.subject
Monoacylglycerol
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Aging Modifies the Enzymatic Activities Involved in 2-Arachidonoylglycerol Metabolism
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.journal.volume
39
dc.journal.number
2
dc.journal.pagination
209-220
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Hoboken
dc.description.fil
Fil: Pascual, Ana Clara. Universidad Nacional del Sur; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto de Investigaciones Bioquímicas Bahía Blanca (i); Argentina
dc.description.fil
Fil: Gaveglio, Virginia Lucía. Universidad Nacional del Sur; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto de Investigaciones Bioquímicas Bahía Blanca (i); Argentina
dc.description.fil
Fil: Giusto, Norma Maria. Universidad Nacional del Sur; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto de Investigaciones Bioquímicas Bahía Blanca (i); Argentina
dc.description.fil
Fil: Pasquaré, Susana Juana. Universidad Nacional del Sur; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto de Investigaciones Bioquímicas Bahía Blanca (i); Argentina
dc.journal.title
Biofactors
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/biof.1055/abstract
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/biof.1055
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/issn/0951-6433
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