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dc.contributor.author
Acosta Rodríguez, Victoria América
dc.contributor.author
Marquez, Sebastian
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Salvador, Gabriela Alejandra
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Pasquaré, Susana Juana
dc.contributor.author
Gorne, Lucas Damián
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Garbarino Pico, Eduardo
dc.contributor.author
Giusto, Norma Maria
dc.contributor.author
Guido, Mario Eduardo
dc.date.available
2016-03-07T14:31:23Z
dc.date.issued
2013-05-02
dc.identifier.citation
Acosta Rodríguez, Victoria América; Marquez, Sebastian; Salvador, Gabriela Alejandra; Pasquaré, Susana Juana; Gorne, Lucas Damián; et al.; Daily rhythms of glycerophospholipid synthesis in fibroblast cultures involve differential enzyme contributions; American Society for Biochemistry and Molecular Biology; Journal of Lipid Research; 54; 7; 2-5-2013; 1798-1811
dc.identifier.issn
0022-2275
dc.identifier.uri
http://hdl.handle.net/11336/4648
dc.description.abstract
Circadian clocks regulate the temporal organization of several biochemical processes including lipid metabolism and their disruption leads to severe metabolic disorders. Immortalized cell lines acting as circadian clocks display daily variations in [32P]-phospholipid labeling; however, the regulation of glycerophospholipid (GPL) synthesis by internal clocks remains unknown. Here we found that arrested NIH 3T3 cells synchronized with a 2 h-serum shock exhibited temporal oscillations in: a) the labeling of total [3H]- GPLs, with lowest levels around 28 and 56 h, and b) the activity of GPL-synthesizing and -remodeling enzymes such as phosphatidate phosphohydrolase 1 (PAP-1) and lysophospholipid acyltransferases (LPLAT) respectively, with antiphase profiles. In addition, we investigated the temporal regulation of phosphatidylcholine (PC) biosynthesis. PC is mainly synthesized through the Kennedy pathway with Choline Kinase (ChoK) and CTP:phosphocholine cytidylyltranferase (CCT) as key regulatory enzymes. We observed that the PC labeling exhibited daily changes with the lowest levels every ~28 h that were accompanied by brief increases in CCT activity and the oscillation in ChoK mRNA expression and activity. Results demonstrate that the metabolisms of GPLs and particularly of PC in synchronized fibroblasts are subject to a complex temporal control involving concerted changes in the expression and/or activities of specific synthesizing enzymes.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Society for Biochemistry and Molecular Biology
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Phospholipid
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Circadian Rhythm
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Fibroblast
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Serum Shock
dc.subject
Synchronization
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Biología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Daily rhythms of glycerophospholipid synthesis in fibroblast cultures involve differential enzyme contributions
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.identifier.eissn
1539-7262
dc.journal.volume
54
dc.journal.number
7
dc.journal.pagination
1798-1811
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Bethesda
dc.description.fil
Fil: Acosta Rodríguez, Victoria América. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina
dc.description.fil
Fil: Marquez, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina
dc.description.fil
Fil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto de Investigaciones Bioquímicas Bahía Blanca (i); Argentina
dc.description.fil
Fil: Pasquaré, Susana Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto de Investigaciones Bioquímicas Bahía Blanca (i); Argentina
dc.description.fil
Fil: Gorne, Lucas Damián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina
dc.description.fil
Fil: Garbarino Pico, Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina
dc.description.fil
Fil: Giusto, Norma Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto de Investigaciones Bioquímicas Bahía Blanca (i); Argentina
dc.description.fil
Fil: Guido, Mario Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina
dc.journal.title
Journal of Lipid Research
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1194/jlr.M034264
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.jlr.org/content/54/7/1798
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3679383/
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/issn/0022-2275
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