Show simple item record

dc.contributor.author Acosta Rodríguez, Victoria América
dc.contributor.author Marquez, Sebastian
dc.contributor.author Salvador, Gabriela Alejandra
dc.contributor.author Pasquaré, Susana Juana
dc.contributor.author Gorne, Lucas Damián
dc.contributor.author Garbarino Pico, Eduardo
dc.contributor.author Giusto, Norma Maria
dc.contributor.author Guido, Mario Eduardo
dc.date.available 2016-03-07T14:31:23Z
dc.date.issued 2013-05-02
dc.identifier.citation Acosta Rodríguez, Victoria América; Marquez, Sebastian; Salvador, Gabriela Alejandra; Pasquaré, Susana Juana; Gorne, Lucas Damián; et al.; Daily rhythms of glycerophospholipid synthesis in fibroblast cultures involve differential enzyme contributions; American Society for Biochemistry and Molecular Biology; Journal of Lipid Research; 54; 7; 2-5-2013; 1798-1811
dc.identifier.issn 0022-2275
dc.identifier.uri http://hdl.handle.net/11336/4648
dc.description.abstract Circadian clocks regulate the temporal organization of several biochemical processes including lipid metabolism and their disruption leads to severe metabolic disorders. Immortalized cell lines acting as circadian clocks display daily variations in [32P]-phospholipid labeling; however, the regulation of glycerophospholipid (GPL) synthesis by internal clocks remains unknown. Here we found that arrested NIH 3T3 cells synchronized with a 2 h-serum shock exhibited temporal oscillations in: a) the labeling of total [3H]- GPLs, with lowest levels around 28 and 56 h, and b) the activity of GPL-synthesizing and -remodeling enzymes such as phosphatidate phosphohydrolase 1 (PAP-1) and lysophospholipid acyltransferases (LPLAT) respectively, with antiphase profiles. In addition, we investigated the temporal regulation of phosphatidylcholine (PC) biosynthesis. PC is mainly synthesized through the Kennedy pathway with Choline Kinase (ChoK) and CTP:phosphocholine cytidylyltranferase (CCT) as key regulatory enzymes. We observed that the PC labeling exhibited daily changes with the lowest levels every ~28 h that were accompanied by brief increases in CCT activity and the oscillation in ChoK mRNA expression and activity. Results demonstrate that the metabolisms of GPLs and particularly of PC in synchronized fibroblasts are subject to a complex temporal control involving concerted changes in the expression and/or activities of specific synthesizing enzymes.
dc.format application/pdf
dc.language.iso eng
dc.publisher American Society for Biochemistry and Molecular Biology
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject PHOSPHOLIPID
dc.subject CIRCADIAN RHYTHM
dc.subject FIBROBLAST
dc.subject SERUM SHOCK
dc.subject SYNCHRONIZATION
dc.subject.classification Biología
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Daily rhythms of glycerophospholipid synthesis in fibroblast cultures involve differential enzyme contributions
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2016-03-30 10:35:44.97925-03
dc.identifier.eissn 1539-7262
dc.journal.volume 54
dc.journal.number 7
dc.journal.pagination 1798-1811
dc.journal.pais Estados Unidos
dc.journal.ciudad Bethesda
dc.description.fil Fil: Acosta Rodríguez, Victoria América. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina
dc.description.fil Fil: Marquez, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina
dc.description.fil Fil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Cientí­ficas y Técnicas. Centro Científico Tecnológico Bahí­a Blanca. Instituto de Investigaciones Bioquímicas Bahí­a Blanca (i); Argentina
dc.description.fil Fil: Pasquaré, Susana Juana. Consejo Nacional de Investigaciones Cientí­ficas y Técnicas. Centro Científico Tecnológico Bahí­a Blanca. Instituto de Investigaciones Bioquímicas Bahí­a Blanca (i); Argentina
dc.description.fil Fil: Gorne, Lucas Damián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina
dc.description.fil Fil: Garbarino Pico, Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina
dc.description.fil Fil: Giusto, Norma Maria. Consejo Nacional de Investigaciones Cientí­ficas y Técnicas. Centro Científico Tecnológico Bahí­a Blanca. Instituto de Investigaciones Bioquímicas Bahí­a Blanca (i); Argentina
dc.description.fil Fil: Guido, Mario Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina
dc.journal.title Journal of Lipid Research
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1194/jlr.M034264
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://www.jlr.org/content/54/7/1798
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3679383/
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/issn/0022-2275


Archivos asociados

Icon
Blocked Acceso no disponible

This item appears in the following Collection(s)

Show simple item record

info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)