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dc.contributor.author
Acosta Rodríguez, Victoria América  
dc.contributor.author
Marquez, Sebastian  
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Salvador, Gabriela Alejandra  
dc.contributor.author
Pasquaré, Susana Juana  
dc.contributor.author
Gorne, Lucas Damián  
dc.contributor.author
Garbarino Pico, Eduardo  
dc.contributor.author
Giusto, Norma Maria  
dc.contributor.author
Guido, Mario Eduardo  
dc.date.available
2016-03-07T14:31:23Z  
dc.date.issued
2013-05-02  
dc.identifier.citation
Acosta Rodríguez, Victoria América; Marquez, Sebastian; Salvador, Gabriela Alejandra; Pasquaré, Susana Juana; Gorne, Lucas Damián; et al.; Daily rhythms of glycerophospholipid synthesis in fibroblast cultures involve differential enzyme contributions; American Society for Biochemistry and Molecular Biology; Journal of Lipid Research; 54; 7; 2-5-2013; 1798-1811  
dc.identifier.issn
0022-2275  
dc.identifier.uri
http://hdl.handle.net/11336/4648  
dc.description.abstract
Circadian clocks regulate the temporal organization of several biochemical processes including lipid metabolism and their disruption leads to severe metabolic disorders. Immortalized cell lines acting as circadian clocks display daily variations in [32P]-phospholipid labeling; however, the regulation of glycerophospholipid (GPL) synthesis by internal clocks remains unknown. Here we found that arrested NIH 3T3 cells synchronized with a 2 h-serum shock exhibited temporal oscillations in: a) the labeling of total [3H]- GPLs, with lowest levels around 28 and 56 h, and b) the activity of GPL-synthesizing and -remodeling enzymes such as phosphatidate phosphohydrolase 1 (PAP-1) and lysophospholipid acyltransferases (LPLAT) respectively, with antiphase profiles. In addition, we investigated the temporal regulation of phosphatidylcholine (PC) biosynthesis. PC is mainly synthesized through the Kennedy pathway with Choline Kinase (ChoK) and CTP:phosphocholine cytidylyltranferase (CCT) as key regulatory enzymes. We observed that the PC labeling exhibited daily changes with the lowest levels every ~28 h that were accompanied by brief increases in CCT activity and the oscillation in ChoK mRNA expression and activity. Results demonstrate that the metabolisms of GPLs and particularly of PC in synchronized fibroblasts are subject to a complex temporal control involving concerted changes in the expression and/or activities of specific synthesizing enzymes.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
American Society for Biochemistry and Molecular Biology  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Phospholipid  
dc.subject
Circadian Rhythm  
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Fibroblast  
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Serum Shock  
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Synchronization  
dc.subject.classification
Biología  
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Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Daily rhythms of glycerophospholipid synthesis in fibroblast cultures involve differential enzyme contributions  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2016-03-30 10:35:44.97925-03  
dc.identifier.eissn
1539-7262  
dc.journal.volume
54  
dc.journal.number
7  
dc.journal.pagination
1798-1811  
dc.journal.pais
Estados Unidos  
dc.journal.ciudad
Bethesda  
dc.description.fil
Fil: Acosta Rodríguez, Victoria América. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina  
dc.description.fil
Fil: Marquez, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina  
dc.description.fil
Fil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Cientí­ficas y Técnicas. Centro Científico Tecnológico Bahí­a Blanca. Instituto de Investigaciones Bioquímicas Bahí­a Blanca (i); Argentina  
dc.description.fil
Fil: Pasquaré, Susana Juana. Consejo Nacional de Investigaciones Cientí­ficas y Técnicas. Centro Científico Tecnológico Bahí­a Blanca. Instituto de Investigaciones Bioquímicas Bahí­a Blanca (i); Argentina  
dc.description.fil
Fil: Gorne, Lucas Damián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina  
dc.description.fil
Fil: Garbarino Pico, Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina  
dc.description.fil
Fil: Giusto, Norma Maria. Consejo Nacional de Investigaciones Cientí­ficas y Técnicas. Centro Científico Tecnológico Bahí­a Blanca. Instituto de Investigaciones Bioquímicas Bahí­a Blanca (i); Argentina  
dc.description.fil
Fil: Guido, Mario Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina  
dc.journal.title
Journal of Lipid Research  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1194/jlr.M034264  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.jlr.org/content/54/7/1798  
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info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3679383/  
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info:eu-repo/semantics/altIdentifier/issn/0022-2275