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dc.contributor.author Demonte, Ana María Magdalena
dc.contributor.author Asención Diez, Matías Damián
dc.contributor.author Naleway, Conrad
dc.contributor.author Iglesias, Alberto Alvaro
dc.contributor.author Ballicora, Miguel A.
dc.date.available 2018-05-29T16:19:13Z
dc.date.issued 2017-01
dc.identifier.citation Demonte, Ana María Magdalena; Asención Diez, Matías Damián; Naleway, Conrad; Iglesias, Alberto Alvaro; Ballicora, Miguel A.; Monofluorophosphate Blocks Internal Polysaccharide Synthesis in Streptococcus mutans; Public Library of Science; Plos One; 12; 1; 1-2017; 1-10
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/11336/46430
dc.description.abstract Streptococcus mutans is the leading cause of dental caries worldwide by accumulating a glycogen-like internal polysaccharide (IPS) that contributes to cariogenicity when sugars are in excess. Sodium monofluorophosphate (MFP) is an active anticariogenic compound in toothpastes. Herein, we show that MFP inhibits (with an I0.5 of 1.5 mM) the S. mutans ADP-glucose pyrophosphorylase (EC 2.7.7.27), which catalyzes the key step in IPS biosynthesis. Enzyme inhibition by MFP is similar to orthophosphate (Pi), except that the effect caused by MFP is not reverted by fructose-1,6-bisP, as occurs with Pi. Inhibition was correlated with a decrease in acidogenesis and IPS accumulation in S. mutans cells cultured with 2 mM sodium MFP. These effects were not mimicked by sodium fluoride. Considering that glycogen synthesis occurs by different pathways in mammals and bacteria, ADP-glucose pyrophosphorylase could be visualized as a molecular target for controlling S. mutans virulence. Our results strongly suggest that MFP is a suitable compound to affect such a target, inducing an anticariogenic effect primarily by inhibiting a key step in IPS synthesis.
dc.format application/pdf
dc.language.iso eng
dc.publisher Public Library of Science
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject ADP-glucose pyrophosphorylase
dc.subject Allosteric regulation
dc.subject Internal polysaccharide
dc.subject Fructose-1,6-bisP
dc.subject Toothpaste
dc.subject Inhibition
dc.subject.classification Otras Ciencias Biológicas
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Monofluorophosphate Blocks Internal Polysaccharide Synthesis in Streptococcus mutans
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2018-05-11T21:17:34Z
dc.journal.volume 12
dc.journal.number 1
dc.journal.pagination 1-10
dc.journal.pais Estados Unidos
dc.journal.ciudad San Francisco
dc.description.fil Fil: Demonte, Ana María Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
dc.description.fil Fil: Asención Diez, Matías Damián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
dc.description.fil Fil: Naleway, Conrad. Loyola University Chicago; Estados Unidos
dc.description.fil Fil: Iglesias, Alberto Alvaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
dc.description.fil Fil: Ballicora, Miguel A.. Loyola University Chicago; Estados Unidos
dc.journal.title Plos One
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1371/journal.pone.0170483
dc.conicet.fuente individual


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    Articulos de INSTITUTO DE AGROBIOTECNOLOGIA DEL LITORAL

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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)