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dc.contributor.author
Demonte, Ana María Magdalena
dc.contributor.author
Asención Diez, Matías Damián
dc.contributor.author
Naleway, Conrad
dc.contributor.author
Iglesias, Alberto Alvaro
dc.contributor.author
Ballicora, Miguel A.
dc.date.available
2018-05-29T16:19:13Z
dc.date.issued
2017-01
dc.identifier.citation
Demonte, Ana María Magdalena; Asención Diez, Matías Damián; Naleway, Conrad; Iglesias, Alberto Alvaro; Ballicora, Miguel A.; Monofluorophosphate Blocks Internal Polysaccharide Synthesis in Streptococcus mutans; Public Library of Science; Plos One; 12; 1; 1-2017; 1-10
dc.identifier.issn
1932-6203
dc.identifier.uri
http://hdl.handle.net/11336/46430
dc.description.abstract
Streptococcus mutans is the leading cause of dental caries worldwide by accumulating a glycogen-like internal polysaccharide (IPS) that contributes to cariogenicity when sugars are in excess. Sodium monofluorophosphate (MFP) is an active anticariogenic compound in toothpastes. Herein, we show that MFP inhibits (with an I0.5 of 1.5 mM) the S. mutans ADP-glucose pyrophosphorylase (EC 2.7.7.27), which catalyzes the key step in IPS biosynthesis. Enzyme inhibition by MFP is similar to orthophosphate (Pi), except that the effect caused by MFP is not reverted by fructose-1,6-bisP, as occurs with Pi. Inhibition was correlated with a decrease in acidogenesis and IPS accumulation in S. mutans cells cultured with 2 mM sodium MFP. These effects were not mimicked by sodium fluoride. Considering that glycogen synthesis occurs by different pathways in mammals and bacteria, ADP-glucose pyrophosphorylase could be visualized as a molecular target for controlling S. mutans virulence. Our results strongly suggest that MFP is a suitable compound to affect such a target, inducing an anticariogenic effect primarily by inhibiting a key step in IPS synthesis.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Public Library of Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Adp-Glucose Pyrophosphorylase
dc.subject
Allosteric Regulation
dc.subject
Internal Polysaccharide
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Fructose-1,6-Bisp
dc.subject
Toothpaste
dc.subject
Inhibition
dc.subject.classification
Otras Ciencias Biológicas
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Monofluorophosphate Blocks Internal Polysaccharide Synthesis in Streptococcus mutans
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-05-11T21:17:34Z
dc.journal.volume
12
dc.journal.number
1
dc.journal.pagination
1-10
dc.journal.pais
Estados Unidos
dc.journal.ciudad
San Francisco
dc.description.fil
Fil: Demonte, Ana María Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
dc.description.fil
Fil: Asención Diez, Matías Damián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
dc.description.fil
Fil: Naleway, Conrad. Loyola University Chicago; Estados Unidos
dc.description.fil
Fil: Iglesias, Alberto Alvaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
dc.description.fil
Fil: Ballicora, Miguel A.. Loyola University Chicago; Estados Unidos
dc.journal.title
Plos One
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1371/journal.pone.0170483


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