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Artículo

Down-regulation of tomato PHYTOL KINASE strongly impairs tocopherol biosynthesis and affects prenyllipid metabolism in an organ-specific manner

Almeida, Juliana; da Silva Azevedo, Mariana; Spicher, Livia; Glauser, Gaetán; vom Dorp, Katharina; Guyer, Luzia; Carranza, Andrea del ValleIcon ; Asis, RamónIcon ; Pereira de Souza, Amanda; Buckeridge, Marcos; Demarco, Diego; Bres, Cécil; Rothan, Christophe; Pereira Peres, Lazaro Eustáquio; Hörtensteiner, Stephan; Kessler, Felix; Dörmann, Peter; Carrari, Fernando OscarIcon ; Rossi, Magdalena
Fecha de publicación: 11/2015
Editorial: Oxford University Press
Revista: Journal of Experimental Botany
ISSN: 0022-0957
e-ISSN: 1460-2431
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Biológicas

Resumen

Tocopherol, a compound with vitamin E (VTE) activity, is a conserved constituent of the plastidial antioxidant network in photosynthetic organisms. The synthesis of tocopherol involves the condensation of an aromatic head group with an isoprenoid prenyl side chain. The latter, phytyl diphosphate, can be derived from chlorophyll phytol tail recycling, which depends on phytol kinase (VTE5) activity. How plants co-ordinate isoprenoid precursor distribution for supplying biosynthesis of tocopherol and other prenyllipids in different organs is poorly understood. Here, Solanum lycopersicum plants impaired in the expression of two VTE5-like genes identified by phylogenetic analyses, named SlVTE5 and SlFOLK, were characterized. Our data show that while SlFOLK does not affect tocopherol content, the production of this metabolite is >80% dependent on SlVTE5 in tomato, in both leaves and fruits. VTE5 deficiency greatly impacted lipid metabolism, including prenylquinones, carotenoids, and fatty acid phytyl esters. However, the prenyllipid profile greatly differed between source and sink organs, revealing organ-specific metabolic adjustments in tomato. Additionally, VTE5-deficient plants displayed starch accumulation and lower CO2 assimilation in leaves associated with mild yield penalty. Taken together, our results provide valuable insights into the distinct regulation of isoprenoid metabolism in leaves and fruits and also expose the interaction between lipid and carbon metabolism, which results in carbohydrate export blockage in the VTE5-deficient plants, affecting tomato fruit quality.
Palabras clave: Carotenoids , Chlorophyll , Phytol , Phytol Kinase , Prenyllipids , Solanum Lycopersicum , Tocopherol , Tomato , Vitamin E
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/46286
URL: https://academic.oup.com/jxb/article/67/3/919/2893353
DOI: http://dx.doi.org/10.1093/jxb/erv504
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Articulos(CIBICI)
Articulos de CENTRO DE INV.EN BIOQUI.CLINICA E INMUNOLOGIA
Citación
Almeida, Juliana; da Silva Azevedo, Mariana; Spicher, Livia; Glauser, Gaetán; vom Dorp, Katharina; et al.; Down-regulation of tomato PHYTOL KINASE strongly impairs tocopherol biosynthesis and affects prenyllipid metabolism in an organ-specific manner; Oxford University Press; Journal of Experimental Botany; 67; 3; 11-2015; 919-934
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