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dc.contributor.author
Ravera, Silvia
dc.contributor.author
Quick, Matthias
dc.contributor.author
Nicola, Juan Pablo
dc.contributor.author
Carrasco, Nancy
dc.contributor.author
Amzel, Mario
dc.date.available
2018-05-24T15:46:52Z
dc.date.issued
2015-05
dc.identifier.citation
Ravera, Silvia; Quick, Matthias; Nicola, Juan Pablo; Carrasco, Nancy; Amzel, Mario; Beyond non-integer Hill coefficients: A novel approach to analyzing binding data, applied to Na + -driven transporters; Rockefeller University Press; Journal Of General Physiology; 145; 6; 5-2015; 555-563
dc.identifier.issn
0022-1295
dc.identifier.uri
http://hdl.handle.net/11336/46077
dc.description.abstract
Prokaryotic and eukaryotic Na(+)-driven transporters couple the movement of one or more Na(+) ions down their electrochemical gradient to the active transport of a variety of solutes. When more than one Na(+) is involved, Na(+)-binding data are usually analyzed using the Hill equation with a non-integer exponent n. The results of this analysis are an overall Kd-like constant equal to the concentration of ligand that produces half saturation and n, a measure of cooperativity. This information is usually insufficient to provide the basis for mechanistic models. In the case of transport using two Na(+) ions, an n < 2 indicates that molecules with only one of the two sites occupied are present at low saturation. Here, we propose a new way of analyzing Na(+)-binding data for the case of two Na(+) ions that, by taking into account binding to individual sites, provides far more information than can be obtained by using the Hill equation with a non-integer coefficient: it yields pairs of possible values for the Na(+) affinities of the individual sites that can only vary within narrowly bounded ranges. To illustrate the advantages of the method, we present experimental scintillation proximity assay (SPA) data on binding of Na(+) to the Na(+)/I(-) symporter (NIS). SPA is a method widely used to study the binding of Na(+) to Na(+)-driven transporters. NIS is the key plasma membrane protein that mediates active I(-) transport in the thyroid gland, the first step in the biosynthesis of the thyroid hormones, of which iodine is an essential constituent. NIS activity is electrogenic, with a 2:1 Na(+)/I(-) transport stoichiometry. The formalism proposed here is general and can be used to analyze data on other proteins with two binding sites for the same substrate.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Rockefeller University Press
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Sodium-Driven Transporters
dc.subject
Sodium/Iodide Symporter
dc.subject
Hill Coefficient
dc.subject
Scintillation Proximity Assay
dc.subject.classification
Otras Ciencias Biológicas
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Beyond non-integer Hill coefficients: A novel approach to analyzing binding data, applied to Na + -driven transporters
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-05-22T21:48:05Z
dc.identifier.eissn
1540-7748
dc.journal.volume
145
dc.journal.number
6
dc.journal.pagination
555-563
dc.journal.pais
Estados Unidos
dc.journal.ciudad
New York
dc.description.fil
Fil: Ravera, Silvia. University of Yale. School of Medicine; Estados Unidos
dc.description.fil
Fil: Quick, Matthias. Columbia University; Estados Unidos
dc.description.fil
Fil: Nicola, Juan Pablo. University of Yale. School of Medicine; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
dc.description.fil
Fil: Carrasco, Nancy. University of Yale. School of Medicine; Estados Unidos
dc.description.fil
Fil: Amzel, Mario. University Johns Hopkins. Dept.of Chemistry; Estados Unidos
dc.journal.title
Journal Of General Physiology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4442788/
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1085%2Fjgp.201511365
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