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dc.contributor.author Orrillo, Alfredo Gastón
dc.contributor.author Ledesma, Pablo
dc.contributor.author Delgado, Osvaldo Daniel
dc.contributor.author Spagna, Giovanni
dc.contributor.author Breccia, Javier Dario
dc.date.available 2018-05-22T14:03:12Z
dc.date.issued 2007-01-04
dc.identifier.citation Orrillo, Alfredo Gastón; Ledesma, Pablo; Delgado, Osvaldo Daniel; Spagna, Giovanni; Breccia, Javier Dario; Cold-active α-l-rhamnosidase from psychrotolerant bacteria isolated from a sub-Antarctic ecosystem; Elsevier Science Inc; Enzyme and Microbial Technology; 40; 2; 4-1-2007; 236-241
dc.identifier.issn 0141-0229
dc.identifier.uri http://hdl.handle.net/11336/45864
dc.description.abstract Psychrotolerant bacteria obtained from sea water and alimentary tracts of benthonic organisms from the sub-Antarctic environment of Tierra del Fuego (Argentina) were screened for cold-active A-l-rhamnosidase production. Only 10 of a total of 140 isolates were positive for A-l-rhamnosidase activity as determined by a qualitative assay; and five of them showed substantial activity at 5 °C. Molecular identification (16S rDNA) of five selected isolates revealed that four of them were closely related to the genus Pseudoalteromonas while the other was identified as Ralstonia pickettii. The Pseudoalteromonas sp. 005NJ strain was selected for further characterization based on the higher relative A-l-rhamnosidase activity at 4 °C. It showed specific growth rates of 0.12 to 0.19 1/h in the temperature range of 1 to 8 °C, and the A-l-rhamnosidase activity was mainly intracellular. The reaction showed optimal pH and temperature values of 6 and 40 °C respectively and maintained 6% activity at 4 °C. The enzyme was found to be thermo-sensitive, presenting a half-live of 4 min at 50 °C. In presence of 12% (v/v) ethanol the activity decreased by approximately 48%. Cold-active A-l-rhamnosidase might be useful for food processing technologies.
dc.format application/pdf
dc.language.iso eng
dc.publisher Elsevier Science Inc
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject Psychrotolerant
dc.subject α-l-Rhamnosidase
dc.subject Pseudoalteromonas
dc.subject Ralstonia
dc.subject.classification Alimentos y Bebidas
dc.subject.classification Otras Ingenierías y Tecnologías
dc.subject.classification INGENIERÍAS Y TECNOLOGÍAS
dc.title Cold-active α-l-rhamnosidase from psychrotolerant bacteria isolated from a sub-Antarctic ecosystem
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2018-04-18T17:41:52Z
dc.journal.volume 40
dc.journal.number 2
dc.journal.pagination 236-241
dc.journal.pais Estados Unidos
dc.description.fil Fil: Orrillo, Alfredo Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil Fil: Ledesma, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil Fil: Delgado, Osvaldo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil Fil: Spagna, Giovanni. Universitá di Catania; Italia
dc.description.fil Fil: Breccia, Javier Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina
dc.journal.title Enzyme and Microbial Technology
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1016/j.enzmictec.2006.04.002
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0141022906002006
dc.conicet.fuente unificacion


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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)