Mostrar el registro sencillo del ítem

dc.contributor.author
Scocchi, A. M.  
dc.contributor.author
Mroginski, Luis Amado  
dc.date.available
2018-05-16T18:42:27Z  
dc.date.issued
2004-12  
dc.identifier.citation
Scocchi, A. M.; Mroginski, Luis Amado; In vitro conservation of apical meristem-tip of Melia azedarach L. (Meliaceae) under slow-growth conditions: (with 1 table & 2 figures); Fundación Romulo Raggio; Phyton - International Journal of Experimental Botany; 73; 12-2004; 137-143  
dc.identifier.issn
0031-9457  
dc.identifier.uri
http://hdl.handle.net/11336/45409  
dc.description.abstract
Studies on in vitro storage of Melia azedarach -"paradise tree"- under slow-growth conditions were carried out to develop an efficient protocol for the conservation of the genetic diversity of the crop. The response to different growth retardation treatments was examined using clone E. In vitro cultures of apical meristem-tips (0.2-0.3 mm in length) could be effectively maintained for 12 months at 4ºC on ¼ MS basal medium supplemented with 0.5 µM BA. Shoot proliferation was observed on 67 % of the recovered apical meristem-tips, after 12 months at 4ºC. Rooting of the regenerated shoots was achieved by reculturing them on MS + 17.5 µM IBA, during 4 days and subsequently transferred to MS medium lacking growth-regulators for 30 days. Complete plants were successfully transferred to the greenhouse.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Fundación Romulo Raggio  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Conservation  
dc.subject
Germplasm  
dc.subject
In Vitro Culture  
dc.subject
Melia Azedarach  
dc.subject
Meristem  
dc.subject.classification
Ciencias de las Plantas, Botánica  
dc.subject.classification
Ciencias Biológicas  
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS  
dc.title
In vitro conservation of apical meristem-tip of Melia azedarach L. (Meliaceae) under slow-growth conditions: (with 1 table & 2 figures)  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-05-09T14:18:03Z  
dc.identifier.eissn
1851-5657  
dc.journal.volume
73  
dc.journal.pagination
137-143  
dc.journal.pais
Argentina  
dc.description.fil
Fil: Scocchi, A. M.. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina  
dc.description.fil
Fil: Mroginski, Luis Amado. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina  
dc.journal.title
Phyton - International Journal of Experimental Botany  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/26fyxh