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dc.contributor.author
Iannone, María Florencia
dc.contributor.author
Rosales, Eliana Paola
dc.contributor.author
Groppa, María Daniela
dc.contributor.author
Benavides, Maria Patricia
dc.date.available
2016-02-29T18:56:40Z
dc.date.issued
2013-11
dc.identifier.citation
Iannone, María Florencia; Rosales, Eliana Paola; Groppa, María Daniela; Benavides, Maria Patricia; H2O2 involvement in polyamines-induced cell death in tobacco leaf discs; Springer; Journal of Plant Growth Regulation; 32; 4; 11-2013; 745-757
dc.identifier.issn
0721-7595
dc.identifier.uri
http://hdl.handle.net/11336/4525
dc.description.abstract
The response of tobacco (Nicotiana tabacum L.) wild-type SR1 leaf discs in terms of reactive oxygen species (ROS) formation and cell death occurrence was evaluated after exposure to the polyamines (PAs) putrescine (Put), spermidine (Spd), and spermine (Spm). Although NADPH oxidase-like enzyme activity was inhibited by all PAs at 3 or 21 h of treatment, H2O2 content increased significantly in a time- and concentration-dependent manner, suggesting that H2O2 accumulation was linked to the activity of other ROS-generating enzymes. Polyamine oxidase (PAO) activity, which increased markedly upon application of Spd or Spm, is a prime candidate for the increased H2O2 accumulation. Except for 0.1 mM Put, which maintained guaiacol peroxidase (GPOX) and catalase (CAT) activities at the same level as the control, the other PA treatments decreased CAT, ascorbate peroxidase, and GPOX activities at 21 h, contributing to the H2O2 increase. Esterase activity and Evans blue staining, two cell death parameters, were negatively affected at 3 h of treatment with 1 mM Spd and with both concentrations of Spm, whereas at 21 h there was an increase in cell death with both concentrations of the three PAs, except for 0.1 mM Put, which did not alter those parameters. The expression of the senescence-associated cysteine protease gene CP1 was measured to monitor senescence, a physiological cell death process. Application of all PAs increased the expression of the gene, except for 0.1 mM Put, which decreased its expression at 21 h. This result was in agreement with the prevention of cell death exerted by Put and evidenced by Evans blue staining, esterase activity, and electrolyte release.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Cell Death
dc.subject
Reactive Oxygen Species
dc.subject
Polyamines
dc.subject
Nicotiana Tabacum
dc.subject
Hydrogen Peroxide
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
H2O2 involvement in polyamines-induced cell death in tobacco leaf discs
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.journal.volume
32
dc.journal.number
4
dc.journal.pagination
745-757
dc.journal.pais
Alemania
dc.journal.ciudad
Berlín
dc.description.fil
Fil: Iannone, María Florencia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; Argentina
dc.description.fil
Fil: Rosales, Eliana Paola. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina
dc.description.fil
Fil: Groppa, María Daniela. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Quimica Biologica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; Argentina
dc.description.fil
Fil: Benavides, Maria Patricia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; Argentina
dc.journal.title
Journal of Plant Growth Regulation
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs00344-013-9341-9
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s00344-013-9341-9
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/issn/0721-7595


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