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dc.contributor.author
Natalini, Paola Marisel
dc.contributor.author
Mateos, Melina Valeria
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Ilincheta, Monica Graciela
dc.contributor.author
Giusto, Norma Maria
dc.date.available
2016-02-29T15:06:13Z
dc.date.issued
2014-06
dc.identifier.citation
Natalini, Paola Marisel; Mateos, Melina Valeria; Ilincheta, Monica Graciela; Giusto, Norma Maria; A novel light-dependent activation of DAGK and PKC in bovine photoreceptor nuclei; Academic Press Ltd-elsevier Science Ltd; Experimental Eye Research; 125; 6-2014; 142-155
dc.identifier.issn
0014-4835
dc.identifier.uri
http://hdl.handle.net/11336/4504
dc.description.abstract
In this work, we describe a selective light-dependent distribution of the lipid kinase 1,2-diacylglycerol kinase (EC 2.7.1.107, DAGK) and the phosphorylated protein kinase C alpha (pPKCalpha) in a nuclear fraction of photoreceptor cells from bovine retinas. A nuclear fraction enriched in small nuclei from photoreceptor cells (PNF), was obtained when a modified nuclear isolation protocol developed by our laboratory was used. We measured and compared DAGK activity as phosphatidic acid (PA) formation in PNF obtained from retinas exposed to light and in retinas kept in darkness using [gamma-32P]ATP or [3H]DAG. In the absence of exogenous substrates and detergents, no changes in DAGK activity were observed. However, when DAGK activity assays were performed in the presence of exogenous substrates, such as stearoyl arachidonoyl glycerol (SAG) or dioleoyl glycerol (DOG), and different detergents (used to make different DAGK isoforms evident), we observed significant light effects on DAGK activity, suggesting the presence of several DAGK isoforms in PNF. Under conditions favoring DAGKzeta activity (DOG, Triton X-100, dioleoyl phosphatidylserine and R59022) we observed an increase in PA formation in PNF from retinas exposed to light with respect to those exposed to darkness. In contrast, under conditions favoring DAGKepsilon (SAG, octylglucoside and R59022) we observed a decrease in its activity. These results suggest different physiological roles of the above-mentioned DAGK isoforms. Western blot analysis showed that whereas light stimulation of bovine retinas increases DAGKzeta nuclear content, it decreases DAGKepsilon and DAGKbeta content in PNF. The role of PIP2-phospholipase C in light-stimulated DAGK activity was demonstrated using U73122. Light was also observed to induce enhanced pPKCalpha content in PNF. The selective distribution of DAGKzeta and epsilon in PNF could be a light-dependent mechanism that in vertebrate retina promotes selective DAG removal and PKC regulation.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Academic Press Ltd-elsevier Science Ltd
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Diacylglycerol Kinase
dc.subject
Protein Kinase C
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Bovine Photoreceptor Nuclei
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Light-Dependent Distribution
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Retina
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Lipid Second Messengers
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Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
A novel light-dependent activation of DAGK and PKC in bovine photoreceptor nuclei
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.journal.volume
125
dc.journal.pagination
142-155
dc.journal.pais
Estados Unidos
dc.description.fil
Fil: Natalini, Paola Marisel. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET Bahía Blanca. Instituto de Investigaciones Bioquímicas Bahía Blanca (i); Argentina
dc.description.fil
Fil: Mateos, Melina Valeria. Universidad Nacional del Sur; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET Bahía Blanca. Instituto de Investigaciones Bioquímicas Bahía Blanca (i); Argentina
dc.description.fil
Fil: Ilincheta, Monica Graciela. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET Bahía Blanca. Instituto de Investigaciones Bioquímicas Bahía Blanca (i); Argentina
dc.description.fil
Fil: Giusto, Norma Maria. Universidad Nacional del Sur; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET Bahía Blanca. Instituto de Investigaciones Bioquímicas Bahía Blanca (i); Argentina
dc.journal.title
Experimental Eye Research
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.exer.2014.06.007
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pubmed/24950064
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.exer.2014.06.007
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