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dc.contributor.author
Grosso, Carla Gisela  
dc.contributor.author
Blariza, María José  
dc.contributor.author
Mougabure Cueto, Gastón Adolfo  
dc.contributor.author
Picollo, Maria Ines  
dc.contributor.author
Garcia, Beatriz Alicia  
dc.date.available
2018-05-09T14:01:54Z  
dc.date.issued
2016-05  
dc.identifier.citation
Grosso, Carla Gisela; Blariza, María José; Mougabure Cueto, Gastón Adolfo; Picollo, Maria Ines; Garcia, Beatriz Alicia; Identification of three cytochrome P450 genes in the Chagas' disease vector Triatoma infestans : Expression analysis in deltamethrin susceptible and resistant populations; Elsevier Science; Infection, Genetics and Evolution; 44; 5-2016; 459-470  
dc.identifier.issn
1567-1348  
dc.identifier.uri
http://hdl.handle.net/11336/44564  
dc.description.abstract
Cytochrome P450 monooxygenases play a predominant role in the metabolism of insecticides. Many insect P450genes have frequently been associated with detoxification processes allowing the insect to become tolerant orresistant to insecticides. The increases of expression of P450 genes at transcriptional level are often consider responsiblefor increasing the metabolism of insecticides and seems to be a common phenomenon in the evolutionof resistance development in insects. As pyrethroid resistance has been detected in Triatoma infestans, it was ofinterest to analyze genes associated with resistance to insecticides such as those encoding for cytochromesP450. With this purpose, the cDNA sequences of three cytochrome P450 genes (CYP4EM7, CYP3085B1, andCYP3092A6) were identified in this species. Primers and specific Taqman probes were designed from these sequencesto determine their expression by quantitative PCR. The mRNA levels of the cytochrome P450 genes identifiedwere determined from total RNA extracted from pools of fat body collected from individuals of differentresistant and susceptible strains of T. infestans, and at different interval times after the topical application ofthe lethal doses 50% (LD50) of deltamethrin on the ventral abdomen of insects belonging to the different populationsanalyzed. It was detected overexpression of the CYP4EM7 gene in the most resistant strain of T. infestans andthe expression of the three cytochrome P450 genes isolated was induced by deltamethrin in the susceptible andresistant populations included in this study. These results suggest that these genes would be involved in the detoxificationof deltamethrin and support the hypothesis that considers to the cytochrome P450 genes of importancein the development of pyrethroid resistance.  
dc.format
application/pdf  
dc.language.iso
spa  
dc.publisher
Elsevier Science  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Triatoma Infestans  
dc.subject
Chagas´ Disease Vector  
dc.subject
P450 Genes  
dc.subject.classification
Otras Ciencias Biológicas  
dc.subject.classification
Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Identification of three cytochrome P450 genes in the Chagas' disease vector Triatoma infestans : Expression analysis in deltamethrin susceptible and resistant populations  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-05-04T14:59:06Z  
dc.journal.volume
44  
dc.journal.pagination
459-470  
dc.journal.pais
Países Bajos  
dc.journal.ciudad
Amsterdam  
dc.description.fil
Fil: Grosso, Carla Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina  
dc.description.fil
Fil: Blariza, María José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina  
dc.description.fil
Fil: Mougabure Cueto, Gastón Adolfo. Ministerio de Salud. Dirección de Enfermedades Transmisibles Por Vectores. Centro de Referencia de Vectores; Argentina  
dc.description.fil
Fil: Picollo, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina  
dc.description.fil
Fil: Garcia, Beatriz Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina  
dc.journal.title
Infection, Genetics and Evolution  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1016/j.meegid.2016.07.027  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1567134816303161