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dc.contributor.author
Porro, Valentina
dc.contributor.author
Pagotto, Romina María del Luján
dc.contributor.author
Harreguy, María Belén
dc.contributor.author
Ramírez, Sofía
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Crispo, Martina
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Santamaría, Clarisa Guillermina
dc.contributor.author
Luque, Enrique Hugo
dc.contributor.author
Rodriguez, Horacio Adolfo
dc.contributor.author
Bollati Fogolín, Mariela
dc.date.available
2018-05-08T20:32:05Z
dc.date.issued
2015-11
dc.identifier.citation
Porro, Valentina; Pagotto, Romina María del Luján; Harreguy, María Belén; Ramírez, Sofía; Crispo, Martina; et al.; Characterization of Oct4-GFP transgenic mice as a model to study the effect of environmental estrogens on the maturation of male germ cells by using flow cytometry; Pergamon-Elsevier Science Ltd; Journal of Steroid Biochemistry and Molecular Biology; 154; 11-2015; 53-61
dc.identifier.issn
0960-0760
dc.identifier.uri
http://hdl.handle.net/11336/44506
dc.description.abstract
Oct4 is involved in regulation of pluripotency during normal development and is down-regulated during formation of postnatal reservoir of germ cells. We propose thatOct4/GFP transgenic mouse, which mimics the endogenous expression pattern of Oct4, could be used as a mammalian model to study the effects of environmental estrogens on the development of male germ cells. Oct4/GFP maturation profile was assessed during postnatal days -PND- 3, 5, 7, 10, 14 and 80, using flow cytometry. Then, we exposed pregnant mothers to 17α-ethinylestradiol (EE2) from day post coitum (dpc) 5 to PND7. Percentage of Oct4/GFP-expressing cells and levels of expression of Oct4/GPF were increased in PND7 after EE2 exposure. These observations were confirmed by analysis of GFP and endogenous Oct4 protein in the seminiferous tubules and by a reduction in epididymal sperm count in adult mice. We introduced Oct4/GFP mouse together with flow cytometry as a tool to evaluate changes in male germ cells development.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Pergamon-Elsevier Science Ltd
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Oct4
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Gfp
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Male Germ Cells
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Flow Cytometry
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Maturation Estrogens
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Otras Ciencias Biológicas
dc.subject.classification
Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Characterization of Oct4-GFP transgenic mice as a model to study the effect of environmental estrogens on the maturation of male germ cells by using flow cytometry
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-05-02T17:38:53Z
dc.journal.volume
154
dc.journal.pagination
53-61
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Porro, Valentina. Instituto Pasteur de Montevideo; Uruguay
dc.description.fil
Fil: Pagotto, Romina María del Luján. Instituto Pasteur de Montevideo; Uruguay
dc.description.fil
Fil: Harreguy, María Belén. Instituto Pasteur de Montevideo; Uruguay
dc.description.fil
Fil: Ramírez, Sofía. Instituto Pasteur de Montevideo; Uruguay
dc.description.fil
Fil: Crispo, Martina. Instituto Pasteur de Montevideo; Uruguay
dc.description.fil
Fil: Santamaría, Clarisa Guillermina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina
dc.description.fil
Fil: Luque, Enrique Hugo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina
dc.description.fil
Fil: Rodriguez, Horacio Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina
dc.description.fil
Fil: Bollati Fogolín, Mariela. Instituto Pasteur de Montevideo; Uruguay
dc.journal.title
Journal of Steroid Biochemistry and Molecular Biology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.jsbmb.2015.06.006
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0960076015300066
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