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dc.contributor.author
Porro, Valentina
dc.contributor.author
Pagotto, Romina María del Luján
dc.contributor.author
Harreguy, María Belén
dc.contributor.author
Ramírez, Sofía
dc.contributor.author
Crispo, Martina
dc.contributor.author
Santamaría, Clarisa Guillermina
dc.contributor.author
Luque, Enrique Hugo
dc.contributor.author
Rodriguez, Horacio Adolfo
dc.contributor.author
Bollati Fogolín, Mariela
dc.date.available
2018-05-08T20:32:05Z
dc.date.issued
2015-11
dc.identifier.citation
Porro, Valentina; Pagotto, Romina María del Luján; Harreguy, María Belén; Ramírez, Sofía; Crispo, Martina; et al.; Characterization of Oct4-GFP transgenic mice as a model to study the effect of environmental estrogens on the maturation of male germ cells by using flow cytometry; Pergamon-Elsevier Science Ltd; Journal of Steroid Biochemistry and Molecular Biology; 154; 11-2015; 53-61
dc.identifier.issn
0960-0760
dc.identifier.uri
http://hdl.handle.net/11336/44506
dc.description.abstract
Oct4 is involved in regulation of pluripotency during normal development and is down-regulated during formation of postnatal reservoir of germ cells. We propose thatOct4/GFP transgenic mouse, which mimics the endogenous expression pattern of Oct4, could be used as a mammalian model to study the effects of environmental estrogens on the development of male germ cells. Oct4/GFP maturation profile was assessed during postnatal days -PND- 3, 5, 7, 10, 14 and 80, using flow cytometry. Then, we exposed pregnant mothers to 17α-ethinylestradiol (EE2) from day post coitum (dpc) 5 to PND7. Percentage of Oct4/GFP-expressing cells and levels of expression of Oct4/GPF were increased in PND7 after EE2 exposure. These observations were confirmed by analysis of GFP and endogenous Oct4 protein in the seminiferous tubules and by a reduction in epididymal sperm count in adult mice. We introduced Oct4/GFP mouse together with flow cytometry as a tool to evaluate changes in male germ cells development.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Pergamon-Elsevier Science Ltd
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Oct4
dc.subject
Gfp
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Male Germ Cells
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Flow Cytometry
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Maturation Estrogens
dc.subject.classification
Otras Ciencias Biológicas
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Characterization of Oct4-GFP transgenic mice as a model to study the effect of environmental estrogens on the maturation of male germ cells by using flow cytometry
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-05-02T17:38:53Z
dc.journal.volume
154
dc.journal.pagination
53-61
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Porro, Valentina. Instituto Pasteur de Montevideo; Uruguay
dc.description.fil
Fil: Pagotto, Romina María del Luján. Instituto Pasteur de Montevideo; Uruguay
dc.description.fil
Fil: Harreguy, María Belén. Instituto Pasteur de Montevideo; Uruguay
dc.description.fil
Fil: Ramírez, Sofía. Instituto Pasteur de Montevideo; Uruguay
dc.description.fil
Fil: Crispo, Martina. Instituto Pasteur de Montevideo; Uruguay
dc.description.fil
Fil: Santamaría, Clarisa Guillermina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina
dc.description.fil
Fil: Luque, Enrique Hugo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina
dc.description.fil
Fil: Rodriguez, Horacio Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina
dc.description.fil
Fil: Bollati Fogolín, Mariela. Instituto Pasteur de Montevideo; Uruguay
dc.journal.title
Journal of Steroid Biochemistry and Molecular Biology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.jsbmb.2015.06.006
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0960076015300066


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