Mostrar el registro sencillo del ítem
dc.contributor.author
Zulian, Sandra Edith
dc.contributor.author
Ilincheta, Monica Graciela
dc.contributor.author
Giusto, Norma Maria
dc.date.available
2018-05-08T19:26:00Z
dc.date.issued
2006-10-02
dc.identifier.citation
Zulian, Sandra Edith; Ilincheta, Monica Graciela; Giusto, Norma Maria; Insulin promotes diacylglycerol kinase activation by different mechanisms in rat cerebral cortex synaptosomes; Wiley-liss, Div John Wiley & Sons Inc; Journal of Neuroscience Research; 84; 5; 2-10-2006; 1012-1019
dc.identifier.issn
0360-4012
dc.identifier.uri
http://hdl.handle.net/11336/44479
dc.description.abstract
The mechanism by which insulin increases diacylglycerol kinase (DAGK) activity has been studied in cerebral cortex (CC) synaptosomes from adult (3–4 months of age) rats. The purpose of this study was to identify the role of phospholipases C and D (PLC and PLD) in DAGK activation by insulin. Neomycin, an inhibitor of PLC phosphatidylinositol‐bisphosphate (PIP2) specific; ethanol, an inhibitor of phosphatidic acid (PA) formation by the promotion of a transphosphatidyl reaction of phosphatidylcholine phospholipase D (PC‐PLD); and DL propranolol, an inhibitor of phosphatidate phosphohydrolase (PAP), were used in this study. Insulin (0.1 μM) shielded an increase in PA synthesis by [32P] incorporation using [γ‐32P]ATP as substrate and endogenous diacylglycerol (DAG) as co‐substrate. This activated synthesis was strongly inhibited either by ethanol or DL propranolol. Pulse chase experiments also showed a PIP2‐PLC activation within 1 min exposure to insulin. When exogenous unsaturated 18:0‐20:4 DAG was present, insulin increased PA synthesis significantly. However, this stimulatory effect was not observed in the presence of exogenous saturated (di‐16:0). In the presence of R59022, a selective DAGK inhibitor, insulin exerted no stimulatory effect on [32P]PA formation, suggesting a strong relationship between increased PA formation by insulin and DAGK activity. These data indicate that the increased synthesis of PA by insulin could be mediated by the activation of both a PC‐PLD pathway to provide DAG and a direct DAGK activation that is associated to the use of 18:0‐20:4 DAG species. PIP2‐PLC activation may contribute at least partly to the insulin effect on DAGK activity.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley-liss, Div John Wiley & Sons Inc
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject.classification
Otras Ciencias Biológicas
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Insulin promotes diacylglycerol kinase activation by different mechanisms in rat cerebral cortex synaptosomes
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-04-18T15:09:25Z
dc.journal.volume
84
dc.journal.number
5
dc.journal.pagination
1012-1019
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Hoboken
dc.description.fil
Fil: Zulian, Sandra Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
dc.description.fil
Fil: Ilincheta, Monica Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
dc.description.fil
Fil: Giusto, Norma Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
dc.journal.title
Journal of Neuroscience Research
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jnr.21010
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/jnr.21010
Archivos asociados