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dc.contributor.author
Cruz, Luis J.
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Tacken, Paul J.
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Zeelenberg, Ingrid S.
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Srinivas, Mangala
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Bonetto, Fernando Jose
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Weigelin, Bettina
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Eich, Christina
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de Vries, I. Jolanda
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Figdor, Carl G.
dc.date.available
2016-02-25T18:00:27Z
dc.date.issued
2014-10
dc.identifier.citation
Cruz, Luis J.; Tacken, Paul J.; Zeelenberg, Ingrid S.; Srinivas, Mangala; Bonetto, Fernando Jose; et al.; Tracking Targeted Bimodal Nanovaccines: Immune Responses and Routing in Cells, Tissue, and Whole Organism; American Chemical Society; Molecular Pharmaceutics; 11; 12; 10-2014; 4299–4313
dc.identifier.issn
1543-8384
dc.identifier.uri
http://hdl.handle.net/11336/4433
dc.description.abstract
Dendritic cells (DCs) are the most potent antigen-presenting cells (APCs), involved in the induction of immunity and currently exploited for antitumor immunotherapies. An optimized noninvasive imaging modality capable of determining and quantifying DC-targeted nanoparticle (NP) trajectories could provide valuable information regarding therapeutic vaccine outcome. Here, targeted poly(d,l-lactide-co-glycolide) nanoparticles (PLGA NPs) recognizing DC receptors were equipped with superparamagnetic iron oxide particles (SPIO) or gold nanoparticles with fluorescently labeled antigen. The fluorescent label allowed for rapid analysis and quantification of DC-specific uptake of targeted PLGA NPs in comparison to uptake by other cells. Transmission electron microscopy (TEM) showed that a fraction of the encapsulated antigen reached the lysosomal compartment of DCs, where SPIO and gold were already partially released. However, part of the PLGA NPs localized within the cytoplasm, as confirmed by confocal microscopy. DCs targeted with NPs carrying SPIO or fluorescent antigen were detected within lymph nodes as early as 1 h after injection by magnetic resonance imaging (MRI). Despite the fact that targeting did not markedly affect PLGA NP biodistribution on organism and tissue level, it increased delivery of NPs to DCs residing in peripheral lymph nodes and resulted in enhanced T cell proliferation. In conclusion, two imaging agents within a single carrier allows tracking of targeted PLGA NPs at the subcellular, cellular, and organismal levels, thereby facilitating the rational design of in vivo targeted vaccination strategies.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Chemical Society
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Imaging
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Nanocarriers
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Biocompatible And Biodegradable Polymers
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Nanomaterials
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Contrast Agents
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Fluorescence
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Magnetic Resonance Imaging
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Dendritic Cells
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Biotecnología relacionada con la Salud
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Biotecnología de la Salud
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dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
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dc.title
Tracking Targeted Bimodal Nanovaccines: Immune Responses and Routing in Cells, Tissue, and Whole Organism
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.journal.volume
11
dc.journal.number
12
dc.journal.pagination
4299–4313
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
Washington
dc.description.fil
Fil: Cruz, Luis J.. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos
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Fil: Tacken, Paul J.. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos
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Fil: Zeelenberg, Ingrid S.. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos
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Fil: Srinivas, Mangala. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos
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Fil: Bonetto, Fernando Jose. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Física del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina
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Fil: Weigelin, Bettina. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Cell Biology; Países Bajos
dc.description.fil
Fil: Eich, Christina. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos
dc.description.fil
Fil: de Vries, I. Jolanda. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos
dc.description.fil
Fil: Figdor, Carl G.. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos
dc.journal.title
Molecular Pharmaceutics
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/abs/10.1021/mp400717r
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info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/DOI:10.1021/mp400717r
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info:eu-repo/semantics/altIdentifier/issn/1543-8384
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