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dc.contributor.author
Alcaraz, Mirta Raquel
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dc.contributor.author
Schwaighofer, Andreas
dc.contributor.author
Kristament, Christian
dc.contributor.author
Ramer, Georg
dc.contributor.author
Brandstetter, Markus
dc.contributor.author
Goicoechea, Hector Casimiro
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dc.contributor.author
Lendl, Bernhard
dc.date.available
2018-05-03T20:28:21Z
dc.date.issued
2015-06
dc.identifier.citation
Alcaraz, Mirta Raquel; Schwaighofer, Andreas; Kristament, Christian; Ramer, Georg; Brandstetter, Markus; et al.; External-Cavity Quantum Cascade Laser Spectroscopy for Mid-IR Transmission Measurements of Proteins in Aqueous Solution; American Chemical Society; Analytical Chemistry; 87; 13; 6-2015; 6980-6987
dc.identifier.issn
0003-2700
dc.identifier.uri
http://hdl.handle.net/11336/44058
dc.description.abstract
In this work, we report mid-IR transmissionmeasurements of the protein amide I band in aqueous solution atlarge optical paths. A tunable external-cavity quantum cascade laser(EC-QCL) operated in pulsed mode at room temperature allowedone to apply a path length of up to 38 μm, which is four times largerthan that applicable with conventional FT-IR spectrometers. Tominimize temperature-induced variations caused by backgroundabsorption of the ν2-vibration of water (HOH-bending) overlappingwith the amide I region, a highly stable temperature control unitwith relative temperature stability within 0.005 °C was developed.An advanced data processing protocol was established to overcomefluctuations in the fine structure of the emission curve that areinherent to the employed EC-QCL due to its mechanical instabilities. To allow for wavenumber accuracy, a spectral calibrationmethod has been elaborated to reference the acquired IR spectra to the absolute positions of the water vapor absorption bands.Employing this setup, characteristic spectral features of five well-studied proteins exhibiting different secondary structures couldbe measured at concentrations as low as 2.5 mg mL−1. This concentration range could previously only be accessed by IRmeasurements in D2O. Mathematical evaluation of the spectral overlap and comparison of second derivative spectra confirmexcellent agreement of the QCL transmission measurements with protein spectra acquired by FT-IR spectroscopy. This provesthe potential of the applied setup to monitor secondary structure changes of proteins in aqueous solution at extended opticalpath lengths, which allow experiments in flow through configuration.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Chemical Society
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Ec-Qcl
dc.subject
Mid-Ir
dc.subject
Infrared
dc.subject
Protein
dc.subject.classification
Otras Ciencias Químicas
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dc.subject.classification
Ciencias Químicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
External-Cavity Quantum Cascade Laser Spectroscopy for Mid-IR Transmission Measurements of Proteins in Aqueous Solution
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-04-27T20:27:11Z
dc.journal.volume
87
dc.journal.number
13
dc.journal.pagination
6980-6987
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
Washington
dc.description.fil
Fil: Alcaraz, Mirta Raquel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
dc.description.fil
Fil: Schwaighofer, Andreas. Technische Universitat Wien; Austria
dc.description.fil
Fil: Kristament, Christian. Technische Universitat Wien; Austria
dc.description.fil
Fil: Ramer, Georg. Technische Universitat Wien; Austria
dc.description.fil
Fil: Brandstetter, Markus. Technische Universitat Wien; Austria
dc.description.fil
Fil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
dc.description.fil
Fil: Lendl, Bernhard. Technische Universitat Wien; Austria
dc.journal.title
Analytical Chemistry
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1021/acs.analchem.5b01738
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/acs.analchem.5b01738
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