Artículo

Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts

Questa, MaríaIcon ; Romorini, LeonardoIcon ; Bluguermann, CarolinaIcon ; Solari, Claudia MaríaIcon ; Neiman, GabrielIcon ; Luzzani, Carlos DanielIcon ; Scassa, Maria Elida; Sevlever, Gustavo; Guberman, Alejandra SoniaIcon ; Miriuka, Santiago GabrielIcon
Fecha de publicación: 01/2016
Editorial: Elsevier Science
Revista: Stem Cell Research
ISSN: 1873-5061
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:

Resumen

Human foreskin fibroblasts were used to generate the iPSC line iPSC-FH2.1 using the EF1a-hSTEMCCA-loxP vector expressing OCT4, SOX2, c-MYC and KLF4, in 5% O2 culture conditions. Stemness was confirmed, as was pluripotency both in vivo and in vitro, in normoxia and hypoxia. Human Embryonic Stem Cell (hESC) line WA-09 and reprogrammed fibroblast primary culture HFF-FM were used as controls.
Palabras clave: Induced Pluripotent Stem Cells , Hypoxia , Human Foreskin Fibroblasts
 
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Atribución-NoComercial-SinDerivadas 2.5 Argentina (CC BY-NC-ND 2.5 AR)
Identificadores
URI: http://hdl.handle.net/11336/43745
DOI: http://dx.doi.org/10.1016/j.scr.2015.12.026
URL: https://www.sciencedirect.com/science/article/pii/S1873506115002068
Colecciones
Articulos(IIB-INTECH)
Articulos de INST.DE INVEST.BIOTECNOLOGICAS - INSTITUTO TECNOLOGICO CHASCOMUS
Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Citación
Questa, María; Romorini, Leonardo; Bluguermann, Carolina; Solari, Claudia María; Neiman, Gabriel; et al.; Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts; Elsevier Science; Stem Cell Research; 16; 2; 1-2016; 300-303
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