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dc.contributor.author
Regalado González, Jose Javier
dc.contributor.author
Carmona Martín, E.
dc.contributor.author
Castro, P.
dc.contributor.author
Moreno, R.
dc.contributor.author
Gil, J.
dc.contributor.author
Encina, C. L.
dc.date.available
2018-04-27T19:36:29Z
dc.date.issued
2015-05
dc.identifier.citation
Regalado González, Jose Javier; Carmona Martín, E.; Castro, P.; Moreno, R.; Gil, J.; et al.; Micropropagation of wild species of the genus Asparagus L. and their interspecific hybrids with cultivated A. officinalis L., and verification of genetic stability using EST-SSRs; Springer; Plant Cell, Tissue and Organ Culture; 121; 2; 5-2015; 501-510
dc.identifier.issn
0167-6857
dc.identifier.uri
http://hdl.handle.net/11336/43715
dc.description.abstract
An efficient micropropagation method forasparagus species was developed in this study. The methodallows the fast cloning of the elite genotypes from differentasparagus species and the interspecific hybrids obtainedfrom these species. Rhizome bud explants were disinfectedusing 3 g l-1 of benomyl and 20 g l-1 of sodium hypochlorite.Then, they were cultured on Asparagus RhizomeBud Medium 3 (ARBM-3) consisting in modified Murashigeand Skoog medium with salts with EDDHA-Fe(85.7 mg l-1) instead of EDTA-Fe and vitamins, supplementedwith 0.3 mg l-1 NAA, 0.1 mg l-1 KIN, 2 mg l-1ancymidol and 6 % sucrose. Results showed that themethod developed produced high disinfection rates(70?95 %). More than 70 % of the explants developedshoots and the rooting rate on ARBM-3 medium was30?45 %. The rooting rate increased to 60?85 % when theunrooted shoots were subjected to an additional cycle ofrooting, reaching 100 % after two cycles of rooting. Themultiplication was achieved through mechanical divisionof rooted shoot clusters growing in ARBM-3. The acclimatizationrate of the micropropagated plantlets was higherthan 90 %. The micropopagated plantlets were screened forsomaclonal variation using 12 expressed sequence tagderived simple sequence repeat markers. The results confirmedthe character ??true to type?? of the plantlets, indicatingthat the method developed in this study can be usedto successfully micropropagate asparagus species.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Rhizome Buds
dc.subject
Rooting
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Molecular Markers
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Somaclonal Variation
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Asparagus Brachyphyllus
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Asparagus Pseudoscaber
dc.subject.classification
Otras Ciencias Biológicas
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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Otras Biotecnología Agropecuaria
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Biotecnología Agropecuaria
dc.subject.classification
CIENCIAS AGRÍCOLAS
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Otras Ciencias Biológicas
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Micropropagation of wild species of the genus Asparagus L. and their interspecific hybrids with cultivated A. officinalis L., and verification of genetic stability using EST-SSRs
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-04-16T14:56:42Z
dc.identifier.eissn
1573-5044
dc.journal.volume
121
dc.journal.number
2
dc.journal.pagination
501-510
dc.journal.pais
Alemania
dc.journal.ciudad
Berlin
dc.description.fil
Fil: Regalado González, Jose Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Consejo Superior de Investigaciones Científicas; España
dc.description.fil
Fil: Carmona Martín, E.. Consejo Superior de Investigaciones Científicas; España
dc.description.fil
Fil: Castro, P.. Genetic Improvement of Fruits and Vegetables Laboratory; Estados Unidos
dc.description.fil
Fil: Moreno, R.. Universidad de Córdoba; España
dc.description.fil
Fil: Gil, J.. Universidad de Córdoba; España
dc.description.fil
Fil: Encina, C. L.. Consejo Superior de Investigaciones Científicas; España
dc.journal.title
Plant Cell, Tissue and Organ Culture
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1007/s11240-015-0720-8
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs11240-015-0720-8
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