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dc.contributor.author Mantegazza, Adriana Rita
dc.contributor.author Barrio, Maria Marcela
dc.contributor.author Moutel, Sandrine
dc.contributor.author Bover, Laura
dc.contributor.author Weck, Markus
dc.contributor.author Brossart, Peter
dc.contributor.author Teillaud, Jean Luc
dc.contributor.author Mordoh, Jose
dc.date.available 2018-04-25T18:58:34Z
dc.date.issued 2004-04
dc.identifier.citation Mantegazza, Adriana Rita; Barrio, Maria Marcela; Moutel, Sandrine; Bover, Laura; Weck, Markus; et al.; CD63 tetraspanin slows down cell migration and translocates to the endosomal-lysosomal-MIICs route after extracellular stimuli in human immature dendritic cells; American Society of Hematology; Blood; 104; 4; 4-2004; 1183-1190
dc.identifier.issn 0006-4971
dc.identifier.uri http://hdl.handle.net/11336/43490
dc.description.abstract We analyzed herein whether members of the tetraspanin superfamily are involved in human immature dendritic cell (DC) functions such as foreign antigen internalization, phagocytosis, and cell migration. We show that CD63, CD9, CD81, CD82, and CD151 are present in immature DCs. Whereas CD9 and CD81 are mostly expressed at the cell surface, CD63 and CD82 are also located in intracellular organelles. Complexes of monoclonal antibody (Mab) FC-5.01-CD63 or Fab-5.01-CD63 were rapidly translocated "outside-in" and followed the endocytic pathway through early endosomes and lysosomes, reaching major histocompatibility complex (MHC) class II-enriched compartments (MIICs) in less than one hour. Internalization of CD63 was also observed during Saccharomyces cerevisiae phagocytosis. Moreover, an association of CD63 with the beta-glycan receptor dectin-1 was observed. Mabs against CD9, CD63, CD81, and CD82 enhanced by 50% the migration induced by the chemokines macrophage inflammatory protein-5 (MIP-5) and MIP-1alpha. Concomitantly, Mabs against CD63 and CD82 diminished the surface expression of CD29, CD11b, CD18, and alpha5 integrins. By immunoprecipitation experiments we found that CD63 associated with integrins CD11b and CD18. These results suggest that CD9, CD63, CD81, and CD82 could play a role in modulating the interactions between immature DCs and their environment, slowing their migratory ability. However, only CD63 would intervene in the internalization of complex antigens.
dc.format application/pdf
dc.language.iso eng
dc.publisher American Society of Hematology
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject DENDRITIC CELLS
dc.subject MONOCLONAL ANTIBODIES
dc.subject.classification Otras Ciencias Biológicas
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title CD63 tetraspanin slows down cell migration and translocates to the endosomal-lysosomal-MIICs route after extracellular stimuli in human immature dendritic cells
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2018-04-10T13:40:56Z
dc.identifier.eissn 1528-0020
dc.journal.volume 104
dc.journal.number 4
dc.journal.pagination 1183-1190
dc.journal.pais Estados Unidos
dc.journal.ciudad Washington
dc.description.fil Fil: Mantegazza, Adriana Rita. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
dc.description.fil Fil: Barrio, Maria Marcela. Fundación Cáncer. Centro de Investigaciones Oncológicas; Argentina
dc.description.fil Fil: Moutel, Sandrine. Inserm; Francia
dc.description.fil Fil: Bover, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Fundación Cáncer. Centro de Investigaciones Oncológicas; Argentina
dc.description.fil Fil: Weck, Markus. University of Tübingen; Alemania
dc.description.fil Fil: Brossart, Peter. Inserm; Francia
dc.description.fil Fil: Teillaud, Jean Luc. Inserm; Francia
dc.description.fil Fil: Mordoh, Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Fundación Cáncer. Centro de Investigaciones Oncológicas; Argentina
dc.journal.title Blood
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://www.bloodjournal.org/content/104/4/1183.long
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1182/blood-2004-01-0104
dc.conicet.fuente unificacion


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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)