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dc.contributor.author Villegas, Liliana Beatriz
dc.contributor.author Amoroso, Maria Julia del R.
dc.contributor.author Castellanos, Lucia Ines
dc.date.available 2018-04-24T20:41:05Z
dc.date.issued 2005-10
dc.identifier.citation Villegas, Liliana Beatriz; Amoroso, Maria Julia del R.; Castellanos, Lucia Ines; Copper tolerant yeasts isolated from polluted area of Argentina; Wiley VCH Verlag; Journal of Basic Microbiology; 45; 5; 10-2005; 381-391
dc.identifier.issn 0233-111X
dc.identifier.uri http://hdl.handle.net/11336/43373
dc.description.abstract Eleven yeasts were isolated from wastewater sediment samples collected from a copper filter mine plant, located in the province of Tucumán, Argentina, and tested for their heavy metal tolerance. Two isolates were selected based on their multiple tolerance to different heavy metals and their copper biosorption capacity was studied. Analysis of the 26S rDNA D1/D2 domain sequences indicates that isolate RCL‐3 showed similarity with Candida sp. and RCL‐11 with Rhodotorula mucilaginosa . Growth performance and copper toxicity of both yeasts were evaluated using YNB‐glucose medium supplemented with 0.1, 0.2, 0.5 and 1 mM of Cu2+ solutions. Candida sp. RCL‐3 was able to grow up to 7 mg ml–1 biomass in the presence of either 0.1 or 0.2 mM Cu2+, and at 0.5 mM Cu2+ growth reached 5.5 mg ml–1. R. mucilaginosa RCL‐11 reached 8 mg ml–1 in the presence of 0.1 mM Cu2+, and values of 6.5 and 5.5 mg ml–1 biomass were obtained at 0.2 and 0.5 mM Cu2+, respectively. Copper accumulation profiles were different: the metal was librated from the intact cells by Candida sp. whereas R. mucilaginosa did not show release from the cells indicating intracellular storage. Specific biosorption of copper by both isolated yeasts showed increase with the initial copper supplied with the medium (up to 11.5 and 8.0 mg Cu g–1 biomass for Candida sp. and R. mucilaginosa , respectively). However, specific biosorption decreased with time.
dc.format application/pdf
dc.language.iso eng
dc.publisher Wiley VCH Verlag
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject COPPER REMOVAL
dc.subject YEASTS
dc.subject.classification Otras Ciencias Biológicas
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Copper tolerant yeasts isolated from polluted area of Argentina
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2018-04-18T17:51:59Z
dc.journal.volume 45
dc.journal.number 5
dc.journal.pagination 381-391
dc.journal.pais Alemania
dc.journal.ciudad Weinheim
dc.description.fil Fil: Villegas, Liliana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil Fil: Amoroso, Maria Julia del R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil Fil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.journal.title Journal of Basic Microbiology
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1002/jobm.200510569
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jobm.200510569
dc.conicet.fuente unificacion


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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)