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dc.contributor.author
Barroso, Paola Andrea
dc.contributor.author
Marco, Jorge Diego
dc.contributor.author
Kato, H.
dc.contributor.author
Tarama, R.
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Rueda, P.
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Cajal, S. P.
dc.contributor.author
Basombrío, Miguel Ángel Manuel
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Korenaga, M.
dc.contributor.author
Taranto, Nestor Juan
dc.contributor.author
Hashiguchi, Y.
dc.date.available
2018-04-24T20:38:52Z
dc.date.issued
2007-12
dc.identifier.citation
Barroso, Paola Andrea; Marco, Jorge Diego; Kato, H.; Tarama, R.; Rueda, P.; et al.; The identification of sandfly species, from an area of Argentina with endemic leishmaniasis, by the PCR-based analysis of the gene coding for 18S ribosomal RNA; Taylor & Francis; Annals of Tropical Medicine and Parasitology; 101; 3; 12-2007; 247-253
dc.identifier.issn
0003-4983
dc.identifier.uri
http://hdl.handle.net/11336/43372
dc.description.abstract
The area around Río Blanco, in the Orán department in the north of the Argentinian province of Salta, is endemic for American tegumentary leishmaniasis. In an attempt to facilitate the identification of the Lutzomyia species in this area, sequences of the gene coding for the 18S ribosomal RNA (rRNA) of sandflies caught in a Shannon trap were explored, by a combination of PCR and analysis of restriction-fragment-length polymorphism (RFLP). The products from the PCR, which employed two primers developed specifically for this study (Lu.18S 1S and Lu.18S AR), were cloned into a commercial vector (pGEM-T Easy) so that their nucleotide sequences could be investigated. In the RFLP analysis, the products of single and double digestion with the AfaI and HapII restriction enzymes were separated by electrophoresis in 3% or 4% agarose. Taken together with the results of a morphological investigation of the flies, the resultant DNA fragment patterns were sufficient to identify most of the sandflies caught as Lu. neivai. Although two other species, Lu. cortelezzii and Lu. sallesi, were collected, they were relatively rare and only identified morphologically. A single digestion of the 18S-rRNA gene sequences with AfaI or HapII appeared sufficient and useful for the identification of Lu. neivai from the north of Salta province, and for several other Lutzomyia species.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Taylor & Francis
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Sandflies
dc.subject
Typing
dc.subject
Rflp-Pcr
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Argentina
dc.subject.classification
Otras Ciencias Biológicas
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Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
The identification of sandfly species, from an area of Argentina with endemic leishmaniasis, by the PCR-based analysis of the gene coding for 18S ribosomal RNA
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-11-03T19:09:06Z
dc.journal.volume
101
dc.journal.number
3
dc.journal.pagination
247-253
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Barroso, Paola Andrea. Kochi University; Japón. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Marco, Jorge Diego. Kochi University; Japón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
dc.description.fil
Fil: Kato, H.. Yamaguchi University; Japón
dc.description.fil
Fil: Tarama, R.. Yamaguchi University; Japón
dc.description.fil
Fil: Rueda, P.. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina
dc.description.fil
Fil: Cajal, S. P.. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina
dc.description.fil
Fil: Basombrío, Miguel Ángel Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
dc.description.fil
Fil: Korenaga, M.. Kochi University; Japón
dc.description.fil
Fil: Taranto, Nestor Juan. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina
dc.description.fil
Fil: Hashiguchi, Y.. Kochi University; Japón
dc.journal.title
Annals of Tropical Medicine and Parasitology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1179/136485907X156988
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.1179/136485907X156988
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