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dc.contributor.author
Clementz, Adriana Laura
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dc.contributor.author
Del Peso, Gonzalo
dc.contributor.author
Canet, Albert
dc.contributor.author
Yori, Juan Carlos
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dc.contributor.author
Valero, Francisco
dc.date.available
2018-04-24T19:41:22Z
dc.date.issued
2016-06
dc.identifier.citation
Clementz, Adriana Laura; Del Peso, Gonzalo; Canet, Albert; Yori, Juan Carlos; Valero, Francisco; Utilization of discard bovine bone as a support for immobilization of recombinant Rhizopus oryzae lipase expressed in Pichia pastoris; American Chemical Society; Biotechnology Progress; 32; 5; 6-2016; 1246-1253
dc.identifier.issn
8756-7938
dc.identifier.uri
http://hdl.handle.net/11336/43322
dc.description.abstract
In this study the possibility of using discard bovine bone as support for immobilization of Rhizopus oryzae lipase expressed in Pichia pastoris was analyzed. Discard bovine bone were milled and then subjected to a chemical treatment with acetone in order to remove lipids and blood traces. Two types of supports were evaluated: bovine bone and calcined bovine bone for 2 hours at 600°C. Supports were characterized by: ICP, SEM, XRD, FTIR, XPS and N2 adsorption isotherms. Calcined bovine bone presented appropriate characteristics for the lipase immobilization due to the removal of collagen: high porosity, large surface area and suitable porous structure. Biocatalysts were prepared with different initial enzyme load. For the equilibrium adsorption studies, the Langmuir isotherm was used to fit the data results.The immobilization occurs in monolayer to a value of 35 UA mg-1.The activities of biocatalysts were tested in transesterification reaction of olive oil. For the enzyme load used in the test, a final yield percentage of 49.6 was achieved after six methanol additions and 180 min of reaction, similar values were obtained using Relizyme as support. Therefore, the bovine bone discard is an economical and appropriate choice for use support immobilization of enzymes
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Chemical Society
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Biocatalysis
dc.subject
Bovine Bone
dc.subject
Lipase Immobilization
dc.subject
Transesterification
dc.subject
Biodiesel
dc.subject.classification
Biotecnología Industrial
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dc.subject.classification
Biotecnología Industrial
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dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
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dc.title
Utilization of discard bovine bone as a support for immobilization of recombinant Rhizopus oryzae lipase expressed in Pichia pastoris
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-04-13T13:56:07Z
dc.journal.volume
32
dc.journal.number
5
dc.journal.pagination
1246-1253
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
Washington
dc.description.fil
Fil: Clementz, Adriana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigaciones en Catálisis y Petroquímica ; Argentina
dc.description.fil
Fil: Del Peso, Gonzalo. Universitat Autònoma de Barcelona; España
dc.description.fil
Fil: Canet, Albert. Universitat Autònoma de Barcelona; España
dc.description.fil
Fil: Yori, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigaciones en Catálisis y Petroquímica ; Argentina
dc.description.fil
Fil: Valero, Francisco. Universitat Autònoma de Barcelona; España
dc.journal.title
Biotechnology Progress
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/btpr.2321
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/btpr.2321
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