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Artículo

Are cytochrome b gene sequencing and polymorphism-specific polymerase chain reaction as reliable as multilocus enzyme electrophoresis for identifying Leishmania spp. from Argentina?

Marco, Jorge DiegoIcon ; Uezato, Hiroshi; Mimori, Tatsuyuki; Barroso, Paola AndreaIcon ; Korenaga, Masataka; Nonaka, Shigeo; Basombrío, Miguel Ángel ManuelIcon ; Taranto, Nestor Juan; Hashiguchi, Yoshihisa
Fecha de publicación: 12/2006
Editorial: American Society of Tropical Medicine and Hygiene
Revista: American Journal of Tropical Medicine and Hygiene
ISSN: 0002-9637
e-ISSN: 1476-1645
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Biológicas

Resumen

Recently, two techniques, polymerase chain reaction (PCR) amplification and sequencing of cytochrome b gene (cyt b gene sequencing) and polymorphism-specific PCR (PS-PCR) were recommended for Leishmania species identification. Before this study, however, the accuracy of these methods had not been tested against the multilocus enzyme electrophoresis, the current gold standard technique on this task. Therefore, a trial was done for the first time to compare the results obtained by these techniques, using 17 Argentinean Leishmania stocks in independent assays. For all the stocks examined, the same results at species level were obtained by the three techniques. Among them, 14 were assigned to L. (Viannia) braziliensis, and three to L. (V.) guyanensis. The two techniques, cyt b gene sequencing and PS-PCR, were able to distinguish between all the proven species responsible for leishmaniases in Argentina. Thus, both techniques were validated and could be used independently for the species designation of Leishmania parasites in the country.
Palabras clave: Citocromo B , Ps-Pcr , Argentina , Lesihmania
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/43278
URL: http://www.ajtmh.org/content/journals/10.4269/ajtmh.2006.75.256#html_fulltext
Colecciones
Articulos(IPE)
Articulos de INST.DE PATOLOGIA EXPERIMENTAL
Citación
Marco, Jorge Diego; Uezato, Hiroshi; Mimori, Tatsuyuki; Barroso, Paola Andrea; Korenaga, Masataka; et al.; Are cytochrome b gene sequencing and polymorphism-specific polymerase chain reaction as reliable as multilocus enzyme electrophoresis for identifying Leishmania spp. from Argentina?; American Society of Tropical Medicine and Hygiene; American Journal of Tropical Medicine and Hygiene; 75; 2; 12-2006; 256-260
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