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Artículo

Differential involvement of rat sperm choline glycerophospholipids and sphingomyelin in capacitation and the acrosomal reaction

Zanetti, Samanta RominaIcon ; Monclus, Maria de Los AngelesIcon ; Rensetti, Daniel Ernesto; Fornes, Miguel WalterIcon ; Aveldaño, Marta IsabelIcon
Fecha de publicación: 12/2010
Editorial: Elsevier France-editions Scientifiques Medicales Elsevier
Revista: Biochimie
ISSN: 0300-9084
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Biológicas

Resumen

Rat spermatozoa main lipid classes and their fatty acids were studied to assess their possible changes in capacitation and the acrosomal reaction (AR), induced in vitro . Capacitation-associated protein tyrosine phosphorylation, and the ef fl ux of 30% of the total cholesterol from gametes to the medium, took place concomitantly with the release of a similar percentage, i.e. , a larger amount, of the total phospholipid, mostly after hydrolysis of the major choline glycerophospholipids (CGP). Main medium lipid metabolites after capacitation were lyso-CGP and polyenoic fatty acids typical of CGP (22:4n-9, 22:5n-6), as free fatty acids (FFA). The AR, induced by a calcium ionophore, resulted in further phospholipid loss, but the produced metabolites remained in the gametes. CGP decrease in AR accounted for some additional FFA and lyso-CGP, but mostly for (22:5n-6-rich) diglycerides. Hydrolysis of sphingomyelins (SM) to ceramides also occurred, mostly affecting species with very long chain polyenoic fatty acids. Quantitatively, CGP and SM were the lipid classes decreasing the most after capacitation and AR, respectively. The massive cholesterol and phospholipid loss from the gametes during capacitation is thus associated with protein phosphorylation, a function that has been located to the sperm tail. The lipid metabolites produced during AR, by accumulating in the gamete heads, could be implicated in sperm e oocyte interactions.
Palabras clave: Acrosome Reaction , Capacitation , Ceramides , Cholesterol , Glycerophospholipids , Sphingomyelins
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
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URI: http://hdl.handle.net/11336/43271
URL: https://www.sciencedirect.com/science/article/pii/S0300908410003019
DOI: http://dx.doi.org/10.1016/j.biochi.2010.08.015
Colecciones
Articulos(IHEM)
Articulos de INST. HISTOLOGIA Y EMBRIOLOGIA DE MEND DR.M.BURGOS
Articulos(INIBIBB)
Articulos de INST.DE INVEST.BIOQUIMICAS BAHIA BLANCA (I)
Citación
Zanetti, Samanta Romina; Monclus, Maria de Los Angeles; Rensetti, Daniel Ernesto; Fornes, Miguel Walter; Aveldaño, Marta Isabel; Differential involvement of rat sperm choline glycerophospholipids and sphingomyelin in capacitation and the acrosomal reaction; Elsevier France-editions Scientifiques Medicales Elsevier; Biochimie; 92; 12; 12-2010; 1886-1894
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