Mostrar el registro sencillo del ítem

dc.contributor.author
Rizo, Gabriela  
dc.contributor.author
Roldan Olarte, Eugenia Mariela  
dc.contributor.author
Miceli, Dora Cristina  
dc.contributor.author
Jimenez, Luis Emanuel  
dc.contributor.author
Alvarez, Rosa Maria Susana  
dc.date.available
2018-04-23T22:43:17Z  
dc.date.issued
2016-08  
dc.identifier.citation
Rizo, Gabriela; Roldan Olarte, Eugenia Mariela; Miceli, Dora Cristina; Jimenez, Luis Emanuel; Alvarez, Rosa Maria Susana; Structural modifications induced by an in vitro maturation process in zona pellucida glycoproteins of bovine oocytes. A Raman microspectroscopy analysis; Royal Society of Chemistry; RSC Advances; 6; 86; 8-2016; 83429-83437  
dc.identifier.issn
2046-2069  
dc.identifier.uri
http://hdl.handle.net/11336/43208  
dc.description.abstract
Oocyte quality is a determinant factor in the efficiency of an in vitro production process. Since the first oocyte–sperm interactions take place in the zona pellucida, the evaluation and characterization of this egg external matrix is of vital importance for the success of the in vitro fertilization process in mammals. Here we evaluate the modifications in the glycoproteins of the zona pellucida of bovine oocytes resulting from in vitro maturation. Using Raman microspectroscopy, subtle spectral differences were straightforwardly related to specific structural changes that could be correlated with known ultrastructural and morphological properties of the zona pellucida. We performed a detailed evaluation of features concerning both the protein backbone and the glycan moieties of the glycoproteins of the zona pellucida from immature and in vitro matured oocytes. Specific spectral differences involving amide I, amide III, and several bands belonging to the sugar residues allowed the characterization of these two stages in oocyte maturation. A molecular rearrangement of the glycoproteins consisting in the exposition of terminal sugar residues that play a key role in sperm recognition while the glycan core is covered by the polypeptide chain was derived from this study. These results demonstrate the efficiency of the Raman microspectroscopy technique to perform a biochemical characterization of the zona pellucida of bovine oocytes. This technique would be useful to discriminate between immature and in vitro matured oocytes and could also provide a basis for the understanding of the changes induced by maturation methods and of their impact on in vitro fertilization.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Royal Society of Chemistry  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Oocyte  
dc.subject
Zona Pellucida  
dc.subject
Raman  
dc.subject.classification
Otras Ciencias Biológicas  
dc.subject.classification
Ciencias Biológicas  
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS  
dc.title
Structural modifications induced by an in vitro maturation process in zona pellucida glycoproteins of bovine oocytes. A Raman microspectroscopy analysis  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-04-23T19:56:48Z  
dc.journal.volume
6  
dc.journal.number
86  
dc.journal.pagination
83429-83437  
dc.journal.pais
Reino Unido  
dc.description.fil
Fil: Rizo, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina  
dc.description.fil
Fil: Roldan Olarte, Eugenia Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina  
dc.description.fil
Fil: Miceli, Dora Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina  
dc.description.fil
Fil: Jimenez, Luis Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina  
dc.description.fil
Fil: Alvarez, Rosa Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina  
dc.journal.title
RSC Advances  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1039/c6ra06243a  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/Content/ArticleLanding/2016/RA/C6RA06243A