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dc.contributor.author
Castro, Camila Andrea
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dc.contributor.author
Vera, Mario
dc.contributor.author
Donati, Edgardo Ruben
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dc.contributor.author
Sand, Wolfgang
dc.date.available
2016-02-19T18:23:41Z
dc.date.issued
2013-10
dc.identifier.citation
Castro, Camila Andrea; Vera, Mario; Donati, Edgardo Ruben; Sand, Wolfgang; Visualization of attachment and colonization of pyrite surfaces by a novel species of Acidianus; Trans Tech Publications; Advanced Materials Research; 825; 10-2013; 70-73
dc.identifier.issn
1022-6680
dc.identifier.uri
http://hdl.handle.net/11336/4306
dc.description.abstract
In this work we have studied the attachment and colonization of pyrite surfaces by the recently isolated thermophilic archaeon Candidatus Acidianus copahuensis (from the geothermal Caviahue-Copahue system, Argentina). Cells pregrown with sulfur, iron(II) or pyrite were tested. In order to characterize the EPS glycoconjugates of this strain, fluorescent lectins were used. Concanavalin A (ConA) gave the best signal and was selected for further studies. Coupons and grains of pyrite were treated with DAPI (to stain attached cells) and TRITC-ConA (to stain polysaccharides from EPS). Pyrite surfaces were imaged by epifluorescence (EFM) and confocal laser microscopy (CLSM). Initial cell attachment to pyrite grains was estimated to be 26%, 35% and 43% when cells were pregrown with sulfur, iron(II) and pyrite as electron donors, respectively. It was observed that the cell adhesion correlated with an increase of EPS production; both processes were favoured when cells were pregrown with pyrite or iron(II). Also the effect of inorganic phosphate (Pi) starvation in the attachment of Acidianus was studied in similar tests using a base medium without Pi. An increase of cell attachment under Pi starvation conditions was detectable.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Trans Tech Publications
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Acidianus
dc.subject
Archaea
dc.subject
Biofilm
dc.subject
Bioleaching
dc.subject
Eps
dc.subject
Thermophiles
dc.subject.classification
Biotecnología Medioambiental
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dc.subject.classification
Biotecnología del Medio Ambiente
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dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
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dc.title
Visualization of attachment and colonization of pyrite surfaces by a novel species of Acidianus
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.journal.volume
825
dc.journal.pagination
70-73
dc.journal.pais
Suiza
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dc.journal.ciudad
Pfaffikon
dc.description.fil
Fil: Castro, Camila Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina
dc.description.fil
Fil: Vera, Mario. Universitat Duisburg - Essen. Aquatische Biotechnologie Biofilm Centre; Alemania
dc.description.fil
Fil: Donati, Edgardo Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina
dc.description.fil
Fil: Sand, Wolfgang. Universitat Duisburg - Essen. Aquatische Biotechnologie Biofilm Centre; Alemania
dc.journal.title
Advanced Materials Research
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.scientific.net/AMR.825.70
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.4028/www.scientific.net/AMR.825.70
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/issn/1022-6680
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