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Artículo

Characterization of internal ribosomal entry sites of Triatoma virus

Czibener, CeciliaIcon ; Alvarez, DiegoIcon ; Scodeller, EduardoIcon ; Gamarnik, Andrea VanesaIcon
Fecha de publicación: 08/2005
Editorial: Society for General Microbiology
Revista: Journal of General Virology
ISSN: 0022-1317
e-ISSN: 1465-2099
Idioma: Inglés
Tipo de recurso: Artículo publicado

Resumen

Triatoma virus (TrV) belongs to a new family of RNA viruses known as Dicistroviridae. Nucleotide sequence comparisons between different dicistroviruses allowed two putative internal ribosomal entry sites (IRESs) in the TrV RNA to be defined: the 5'UTR IRES of 548 nt and the intergenic region (IGR) IRES of 172 nt. Using monocistronic and bicistronic RNAs, it was shown that the TrV genome contains two functional IRESs that mediate translation initiation in a cap-independent manner. In addition, it was found that the two TrV IRESs were able to direct efficient translation of reporter genes in microinjected Xenopus oocytes, suggesting minimum requirements for host factors. The IGR IRES begins with a non-canonical CUC; however, mutations of this triplet to AUG or CCU did not impair IRES function, indicating that the CUC is not essential for the initiation process. Furthermore, translation efficiency from two TrV IRESs was differentially modulated by IFN-alpha and viral infection.
Palabras clave: Triatoma Virus
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/43041
URL: http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.80842-0
DOI: http://dx.doi.org/10.1099/vir.0.80842-0
Colecciones
Articulos(IIB-INTECH)
Articulos de INST.DE INVEST.BIOTECNOLOGICAS - INSTITUTO TECNOLOGICO CHASCOMUS
Articulos(IIBBA)
Articulos de INST.DE INVEST.BIOQUIMICAS DE BS.AS(I)
Citación
Czibener, Cecilia; Alvarez, Diego; Scodeller, Eduardo; Gamarnik, Andrea Vanesa; Characterization of internal ribosomal entry sites of Triatoma virus; Society for General Microbiology; Journal of General Virology; 86; 8; 8-2005; 2275-2280
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