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dc.contributor.author
Peirú, Salvador  
dc.contributor.author
Aguirre, Andres  
dc.contributor.author
Eberhardt, Maria Florencia  
dc.contributor.author
Braia, Mauricio Javier  
dc.contributor.author
Cabrera, Rodolfo Ariel  
dc.contributor.author
Menzella, Hugo Gabriel  
dc.date.available
2018-04-20T17:58:27Z  
dc.date.issued
2015-12  
dc.identifier.citation
Peirú, Salvador; Aguirre, Andres; Eberhardt, Maria Florencia; Braia, Mauricio Javier; Cabrera, Rodolfo Ariel; et al.; An industrial scale process for the enzymatic removal of steryl glucosides from biodiesel; BioMed Central; Biotechnology For Biofuels; 8; 12-2015; 1-12  
dc.identifier.issn
1754-6834  
dc.identifier.uri
http://hdl.handle.net/11336/42879  
dc.description.abstract
Background: Biodiesels produced from transesterification of vegetable oils have a major quality problem due to thepresence of precipitates, which need to be removed to avoid clogging of filters and engine failures. These precipitateshave been reported to be mostly composed of steryl glucosides (SGs), but so far industrial cost-effective methods toremove these compounds are not available. Here we describe a novel method for the efficient removal of SGs frombiodiesel, based on the hydrolytic activity of a thermostable β-glycosidase obtained from Thermococcus litoralis.Results: A steryl glucosidase (SGase) enzyme from T. litoralis was produced and purified from Escherichia coli culturesexpressing a synthetic gene, and used to treat soybean-derived biodiesel. Several optimization steps allowed for theselection of optimal reaction conditions to finally provide a simple and efficient process for the removal of SGs fromcrude biodiesel. The resulting biodiesel displayed filterability properties similar to distilled biodiesel according to thetotal contamination (TC), the cold soak filtration test (CSFT), filter blocking tendency (FBT), and cold soak filter blockingtendency (CSFBT) tests. The process was successfully scaled up to a 20 ton reactor, confirming its adaptability toindustrial settings.Conclusions: The results presented in this work provide a novel path for the removal of steryl glucosides from biodieselusing a cost-effective, environmentally friendly and scalable enzymatic process, contributing to the adoption ofthis renewable fuel.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
BioMed Central  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Synthetic Biology  
dc.subject
Green Chemistry  
dc.subject
Biofuels  
dc.subject.classification
Biotecnología Industrial  
dc.subject.classification
Biotecnología Industrial  
dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS  
dc.title
An industrial scale process for the enzymatic removal of steryl glucosides from biodiesel  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-04-17T13:50:02Z  
dc.journal.volume
8  
dc.journal.pagination
1-12  
dc.journal.pais
Reino Unido  
dc.journal.ciudad
Londres  
dc.description.fil
Fil: Peirú, Salvador. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Keclon; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Aguirre, Andres. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Keclon; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Eberhardt, Maria Florencia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Braia, Mauricio Javier. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Cabrera, Rodolfo Ariel. Unitec Bio; Argentina  
dc.description.fil
Fil: Menzella, Hugo Gabriel. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Keclon; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.journal.title
Biotechnology For Biofuels  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1186/s13068-015-0405-x  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-015-0405-x