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dc.contributor.author
Santamaria, Miguel H.
dc.contributor.author
Perez Caballero, Eugenia
dc.contributor.author
Corral, Ricardo Santiago
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dc.date.available
2018-04-18T17:20:24Z
dc.date.issued
2015-11
dc.identifier.citation
Santamaria, Miguel H.; Perez Caballero, Eugenia; Corral, Ricardo Santiago; Unmethylated CpG motifs in Toxoplasma gondii DNA induce TLR9- and IFN-β-dependent expression of α-defensin-5 in intestinal epithelial cells; Cambridge University Press; Parasitology; 143; 1; 11-2015; 60-68
dc.identifier.issn
0031-1820
dc.identifier.uri
http://hdl.handle.net/11336/42490
dc.description.abstract
The gut epithelial barrier is a strategic place to prevent, or at least to limit, parasite dissemination upon oral infection with Toxoplasma gondii. Innate immunity to this pathogen results from delicate interactions involving different components of the infecting agent and the host. We herein aimed to examine the molecular mechanism by which protozoan DNA boosts the production of α-defensin-5 (DEFA-5), the main antimicrobial peptide at the target site of infection. The present study shows that DEFA-5 is rapidly upregulated in intestinal epithelial cells following intracellular Toll-like receptor 9 (TLR9) activation by unmethylated CpG motifs in DNA from T. gondii (CpG-DNA). Concomitantly, CpG-DNA purified from the pathogen markedly increased TLR9 mRNA expression levels in the Caco-2 cell line. We further verified that DEFA-5 production was dependent on interferon-β released from these cells upon treatment with CpG-DNA prepared from tachyzoites. Our results suggest that, in protozoan DNA-stimulated intestinal epithelial cells, the TLR9/interferon-β/DEFA-5 pathway may initiate an innate anti-T. gondii response without the need of parasite invasion. These findings highlight the key role of the gut epithelium in Toxoplasma recognition and amplification of local host defence against this microbe, thereby contributing to gain insight into immunoprotective mechanisms and to improve therapeutic strategies.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Cambridge University Press
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Toxoplasma Gondii
dc.subject
Alpha Defensin
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Cpg Dna
dc.subject
Toll-Like Receptor 9
dc.subject.classification
Inmunología
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dc.subject.classification
Medicina Básica
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dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
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dc.title
Unmethylated CpG motifs in Toxoplasma gondii DNA induce TLR9- and IFN-β-dependent expression of α-defensin-5 in intestinal epithelial cells
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-04-17T19:57:39Z
dc.journal.volume
143
dc.journal.number
1
dc.journal.pagination
60-68
dc.journal.pais
Reino Unido
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dc.journal.ciudad
Cambridge
dc.description.fil
Fil: Santamaria, Miguel H.. Centro de Estudios Metabólicos; España
dc.description.fil
Fil: Perez Caballero, Eugenia. Centro de Estudios Metabólicos; España
dc.description.fil
Fil: Corral, Ricardo Santiago. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños ; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Parasitology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1017/S0031182015001456
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/parasitology/article/unmethylated-cpg-motifs-in-toxoplasma-gondii-dna-induce-tlr9-and-ifndependent-expression-of-defensin5-in-intestinal-epithelial-cells/D0F9A3FDEBE962D8C9480440501AC7FA
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