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dc.contributor.author
Lopez Alvarez, Miriam
dc.contributor.author
López Puente, Vanesa
dc.contributor.author
Rodriguez Valencia, Cosme
dc.contributor.author
Angelome, Paula Cecilia
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dc.contributor.author
Liz Marzan, Luis M
dc.contributor.author
Serra, Julia
dc.contributor.author
Pastoriza Santos, Isabel
dc.contributor.author
Gonzalez, Pio
dc.date.available
2018-04-17T19:30:51Z
dc.date.issued
2018-02
dc.identifier.citation
Lopez Alvarez, Miriam; López Puente, Vanesa; Rodriguez Valencia, Cosme; Angelome, Paula Cecilia; Liz Marzan, Luis M; et al.; Osteogenic effects of simvastatin-loaded mesoporous titania thin films; IOP Publishing; Biomedical Materials; 13; 2; 2-2018; 1-24
dc.identifier.issn
1748-6041
dc.identifier.uri
http://hdl.handle.net/11336/42364
dc.description.abstract
The use of statins in the field of bone regeneration is under current investigation due to the existing demand for non-toxic anabolic agents capable of enhancing bone formation in cases of substantial loss. Simvastatin, a coenzyme currently prescribed in clinics to inhibit cholesterol biosynthesis, has been proven to promote osteogenic differentiation by stimulating bone formation and inhibiting osteoclasts activity. We present the loading of simvastatin in mesoporous TiO2 thin films toward combining the pro-osteogenic properties of this molecule with the demonstrated bioactivity of titania. TiO2 thin films processing and characterization were carried out, as well as evaluation of MC3T3-E1 pre-osteoblasts viability when directly incubated with different concentrations of simvastatin, followed by the analysis of osteogenic activity promoted by simvastatin upon loading in the thin films. The accessible porosity of 36% quantified on the 95 ± 5 nm thick mesoporous thin films, together with pore diameters of 5.5 nm, necks between pores of 2.8 nm and interpore distances of 12 ± 2 nm allow the loading of the simvastatin molecule, as confirmed by FTIR spectroscopy. Simvastatin was found to promote MC3T3-E1 pre-osteoblasts viability at concentrations ≤0.01 g l−1, with a cytotoxicity threshold of 0.05 g l−1. We additionally found that film loadings with 0.001 g l−1 simvastatin promotes statistically higher MC3T3-E1 pre-osteoblast proliferation whereas a higher concentration of 0.01 g l−1 leads to statistically higher osteogenic activity (ALP synthesis), after 21 days of incubation, as compared to unloaded films. These results demonstrate the potential of simvastatin local administration based on bioactive mesoporous thin films to promote pro-osteogenic properties. By focusing this strategy on the coating of metallic prostheses, the supply of simvastatin to the target tissue can be favored and risks of systemic side effects will be reduced while enhancing the osteointegration of the implants.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
IOP Publishing
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dc.rights
info:eu-repo/semantics/embargoedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Simvastatin
dc.subject
Mesoporous Titania
dc.subject
Thin Films
dc.subject
Drug Delivery
dc.subject.classification
Nano-materiales
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dc.subject.classification
Nanotecnología
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dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
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dc.title
Osteogenic effects of simvastatin-loaded mesoporous titania thin films
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-04-06T13:52:55Z
dc.journal.volume
13
dc.journal.number
2
dc.journal.pagination
1-24
dc.journal.pais
Reino Unido
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dc.journal.ciudad
Londres
dc.description.fil
Fil: Lopez Alvarez, Miriam. Universidad de Vigo; España
dc.description.fil
Fil: López Puente, Vanesa. Universidad de Vigo; España
dc.description.fil
Fil: Rodriguez Valencia, Cosme. Universidad de Vigo; España
dc.description.fil
Fil: Angelome, Paula Cecilia. Comisión Nacional de Energía Atómica. Centro Atómico Constituyentes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Liz Marzan, Luis M. Ikerbasque; España
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Fil: Serra, Julia. Universidad de Vigo; España
dc.description.fil
Fil: Pastoriza Santos, Isabel. Universidad de Vigo; España
dc.description.fil
Fil: Gonzalez, Pio. Universidad de Vigo; España
dc.journal.title
Biomedical Materials
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dc.rights.embargoDate
2018-09-01
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://iopscience.iop.org/article/10.1088/1748-605X/aa95f1
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1088/1748-605X/aa95f1
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