Artículo
Enhanced extracellular production of trans-resveratrol in Vitis vinifera suspension cultured cells by using cyclodextrins and methyljasmonate
Fecha de publicación:
07/09/2011
Editorial:
Springer
Revista:
Plant Cell Reports
ISSN:
0721-7714
e-ISSN:
1432-203X
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
In this work, the effect of different inducing factors on trans-resveratrol extracellular production in Monastrell grapevine suspension cultured cells is evaluated. A detailed analysis provides the optimal concentrations of cyclodextrins, methyljasmonate and UV irradiation dosage, optimal cell density, elicitation time and sucrose content in the culture media. The results indicate that trans-resveratrol production decreases as the initial cell density increases for a constant elicitor concentration in Monastrell suspension cultured cells treated with cyclodextrins individually or in combination with methyljasmonate, the decrease observed in cell cultures elicited with cyclodextrins alone being far more drastic than those observed in the combined treatment. trans-Resveratrol extracellular production observed by the joint use of cyclodextrins and methyljasmonate (1447.8 ± 60.4 μmol trans-resveratrol g-1 dry weight) is lower when these chemical compounds are combined with UV light short exposure (669.9 ± 45.2 μmol trans-resveratrol g-1 dry weight). Likewise, trans-resveratrol production is dependent on levels of sucrose in the elicitation medium being the maximal levels observed with 20 g l-1 sucrose and the joint action of cyclodextrins and 100 µM methyljasmonate. The sucrose concentration did not seem limiting the process although significantly affect the specific productivity since the lowest sucrose concentration is (10 g l-1), the highest productivity is reached (100.7 ± 5.8 μmol trans-resveratrol g-1 dry weight g-1 sucrose) using cyclodextrins and 25 µM methyljasmonate.
Palabras clave:
Resveratrol
,
Cell Cultures
,
Elicitors
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(IBAM)
Articulos de INST.DE BIOLOGIA AGRICOLA DE MENDOZA
Articulos de INST.DE BIOLOGIA AGRICOLA DE MENDOZA
Citación
Belchi Navarro, Sarai; Almagro, Lorena; Lijavetzky, Diego Claudio; Bru, Roque; Pedreño, María A.; Enhanced extracellular production of trans-resveratrol in Vitis vinifera suspension cultured cells by using cyclodextrins and methyljasmonate; Springer; Plant Cell Reports; 31; 1; 7-9-2011; 81-89
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