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Artículo

2-iodohexadecanal Inhibits thyroid cell growth in part through the induction of let-7f microRNA

Salvarredi, Leonardo AndresIcon ; Thomasz, LisaIcon ; Rossich, Luciano EstebanIcon ; Saiselet, Manuel; Pisarev, Mario AlbertoIcon ; Fusco, Alfredo; Juvenal, Guillermo JuanIcon
Fecha de publicación: 10/2015
Editorial: Elsevier Ireland
Revista: Molecular and Cellular Endocrinology
ISSN: 0303-7207
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Bioquímica y Biología Molecular

Resumen

It is well known that pituitary TSH exerts the major task in the regulation of thyroid function. However, this gland is capable of certain degree of autonomy, independently of TSH control. Iodine plays an important role in thyroid physiology and biochemistry. The thyroid is capable of producing different iodolipids such as 2-iodohexadecanal (2-IHDA). It was shown that this iodolipid mimic some of the inhibitory effects of excess iodide on several thyroid parameters. Objectives: To identify the miRNAs regulated by 2-IHDA in rat thyroid cells and likely characterize their role in thyroid cell proliferation and function. Results: FRTL-5 cells were grown in the presence of TSH and treated with 2-IHDA. Among the miRNAs up-regulated by 2-IHDA we focused on miR-let-7f and miR-138. When we transfected the miRNAs, miR-let-7f but not miR-138 overexpression inhibited proliferation of FRTL 5 cells, while miR-let-7f inhibition restored cell growth in 2-IHDA treated cultures. Analysis of cell cycle by flow cytometric DNA analysis revealed that miR-let-7f inhibition reduced the percentage of 2-IHDA treated cells in G1 phase and an increased of the percentage of cells in S phase was observed upon anti-let-7f transfection. The expresion of Cyclin D1 and Cyclin D3 were reduced after the transfection of miR-let-7f and miR-138, respectively. In in vivo studies we observed that miR-let-7f and miR-138 were up regulated by 2-IHDA during goiter involution. Conclusion: These results suggest that the inhibitory effects of 2-IHDA on FRTL-5 thyroid cell proliferation are mediated in part through the induction of let-7f microRNA.
Palabras clave: Tiroides , Micrornas , Iodolípidos , Cáncer
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Atribución-NoComercial-SinDerivadas 2.5 Argentina (CC BY-NC-ND 2.5 AR)
Identificadores
URI: http://hdl.handle.net/11336/42259
DOI: http://dx.doi.org/10.1016/j.mce.2015.07.007
URL: https://www.sciencedirect.com/science/article/pii/S0303720715300162
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Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Citación
Salvarredi, Leonardo Andres; Thomasz, Lisa; Rossich, Luciano Esteban; Saiselet, Manuel; Pisarev, Mario Alberto; et al.; 2-iodohexadecanal Inhibits thyroid cell growth in part through the induction of let-7f microRNA; Elsevier Ireland; Molecular and Cellular Endocrinology; 414; 10-2015; 224-232
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