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dc.contributor.author
Bandini, Giulia
dc.contributor.author
Mariño, Karina Valeria
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dc.contributor.author
Sampaio Guther, Maria Lucia
dc.contributor.author
Wernimont, Amy K.
dc.contributor.author
Kuettel, Sabine
dc.contributor.author
Qiu, Wei
dc.contributor.author
Afzal, Shamshad
dc.contributor.author
Kelner, Anna
dc.contributor.author
Hui, Raymond
dc.contributor.author
Ferguson, Michael
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dc.date.available
2018-04-15T12:11:28Z
dc.date.issued
2012-07
dc.identifier.citation
Bandini, Giulia; Mariño, Karina Valeria; Sampaio Guther, Maria Lucia; Wernimont, Amy K.; Kuettel, Sabine; et al.; Phosphoglucomutase is absent in Trypanosoma brucei and redundantly substituted by phosphomannomutase and phospho-N-acetylglucosamine mutase; Wiley Blackwell Publishing, Inc; Molecular Microbiology; 85; 3; 7-2012; 513-534
dc.identifier.issn
0950-382X
dc.identifier.uri
http://hdl.handle.net/11336/42071
dc.description.abstract
The enzymes phosphomannomutase (PMM), phospho-N-acetylglucosamine mutase (PAGM) and phosphoglucomutase (PGM) reversibly catalyze the transfer of phosphate between the C6 and C1 hydroxyl groups of mannose, N-acetylglucosamine and glucose, respectively. Although genes for a candidate phosphomannomutase and a phospho-N-acetylglucosamine mutase enzymes have been found in the Trypanosoma brucei genome there is, surprisingly, no candidate gene for phosphoglucomutase. The TbPMM and TbPAGM genes were cloned and expressed in Escherichia coli and the TbPMM enzyme was crystallized and its structure solved at 1.85Å resolution. Antibodies to the recombinant proteins localized endogenous TbPMM to glycosomes in the bloodstream form of the parasite while TbPAGM localized to both the cytosol and glycosomes. Both recombinant enzymes were able to interconvert glucose-phosphates, as well as acting on their own definitive substrates. Analysis of sugar nucleotide levels in parasites with TbPMM or TbPAGM knocked down by RNA interference (RNAi) suggests that, in vivo, PGM activity is catalyzed by both enzymes. This is the first example in any organism of PGM activity being completely replaced in this way and it explains why, uniquely, T. brucei has been able to lose its PGM gene. The RNAi data for TbPMM also showed that this is an essential gene for parasite growth.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Phosphomannomutase
dc.subject
Phophoglucomutase Hosphoglucomutase
dc.subject
Phospho-N-Acetylglucosamine Mutase
dc.subject
Trypanosoma Brucei
dc.subject
Sugar Nucleotide Metabolism
dc.subject.classification
Biología Celular, Microbiología
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.subject.classification
Bioquímica y Biología Molecular
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dc.subject.classification
Medicina Básica
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dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
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dc.title
Phosphoglucomutase is absent in Trypanosoma brucei and redundantly substituted by phosphomannomutase and phospho-N-acetylglucosamine mutase
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-04-09T18:55:00Z
dc.identifier.eissn
1365-2958
dc.journal.volume
85
dc.journal.number
3
dc.journal.pagination
513-534
dc.journal.pais
Reino Unido
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dc.journal.ciudad
Oxford
dc.description.fil
Fil: Bandini, Giulia. University of Dundee; Reino Unido
dc.description.fil
Fil: Mariño, Karina Valeria. University of Dundee; Reino Unido. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil
Fil: Sampaio Guther, Maria Lucia. University of Dundee; Reino Unido
dc.description.fil
Fil: Wernimont, Amy K.. University of Toronto; Canadá
dc.description.fil
Fil: Kuettel, Sabine. University of Dundee; Reino Unido
dc.description.fil
Fil: Qiu, Wei. University of Toronto; Canadá
dc.description.fil
Fil: Afzal, Shamshad. University of Dundee; Reino Unido
dc.description.fil
Fil: Kelner, Anna. University of Dundee; Reino Unido
dc.description.fil
Fil: Hui, Raymond. University of Toronto; Canadá
dc.description.fil
Fil: Ferguson, Michael. University of Dundee; Reino Unido
dc.journal.title
Molecular Microbiology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-2958.2012.08124.x
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/j.1365-2958.2012.08124.x
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/pmid/22676716
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22676716/
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