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dc.contributor.author
Fariña, Julia Ines
dc.contributor.author
Viñarta, Silvana Carolina
dc.contributor.author
Cattaneo, Mauricio
dc.contributor.author
Figueroa L.I.C.
dc.date.available
2018-04-13T19:17:20Z
dc.date.issued
2009-01
dc.identifier.citation
Fariña, Julia Ines; Viñarta, Silvana Carolina; Cattaneo, Mauricio; Figueroa L.I.C.; Structural stability of Sclerotium rolfsii ATCC 201126 β-glucan with fermentation time: a chemical, infrared spectroscopic and enzymatic approach; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 106; 1; 1-2009; 221-232
dc.identifier.issn
1364-5072
dc.identifier.uri
http://hdl.handle.net/11336/42040
dc.description.abstract
Sclerotium rolfsii ATCC 201126 exopolysaccharides (EPSs) recovered at 48 h (EPS I) and 72 h (EPS II) of fermentation, with differences in rheological parameters, hydrogel topography, salt tolerance, antisyneresis, emulsifying and suspending properties, were subjected to a polyphasic characterization in order to detect structural divergences. Methods and Results: Fermenter-scale production led to productivity (Pr) and yield (YP ⁄ C) values higher at 48 h (Pr = 0Æ542 g l)1 h)1 ; YP ⁄ C = 0Æ74) than at 72 h (Pr = 0Æ336 g l)1 h)1 ; YP ⁄ C = 0Æ50). Both EPSs were neutral glucosehomopolysaccharides with a b-(1,3)-glycosidic backbone and single b-(1,6)- glucopyranosyl sidechains regularly attached every three residues in the main chain, as revealed by chemical analyses. The infra-red diagnostic peak at 890 cm)1 confirmed b-glycosidic linkages, while gentiobiose released by b-(1,3)-glucanases confirmed single b-1,6-glycosidic branching for both EPSs. Conclusions: The true modular repeating unit of S. rolfsii ATCC 201126 scleroglucan could be resolved. Structural stability was corroborated and no structural differences could be detected as to account for the variations in EPSs behaviour. Significance and Impact of the Study: Recovery of S. rolfsii ATCC 201126 scleroglucan at 48 h might be considered based on better fermentation kinetic parameters and no detrimental effects on EPS structural features.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Sclerotium Rolfsii
dc.subject
Scleroglucan
dc.subject
Fermentation
dc.subject
Structure
dc.subject
Enzymatic Digestion
dc.subject
Ft-Ir
dc.subject.classification
Otras Ciencias Químicas
dc.subject.classification
Ciencias Químicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Structural stability of Sclerotium rolfsii ATCC 201126 β-glucan with fermentation time: a chemical, infrared spectroscopic and enzymatic approach
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-04-10T14:09:33Z
dc.journal.volume
106
dc.journal.number
1
dc.journal.pagination
221-232
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil
Fil: Viñarta, Silvana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil
Fil: Cattaneo, Mauricio. Universidad Nacional de Tucumán; Argentina
dc.description.fil
Fil: Figueroa L.I.C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.journal.title
Journal of Applied Microbiology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2008.03995.x
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1111/j.1365-2672.2008.03995.x
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