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dc.contributor.author
Martinez, Maria Alejandra
dc.contributor.author
Delgado, Osvaldo Daniel
dc.contributor.author
Baigori, Mario Domingo
dc.contributor.author
Siñeriz, Faustino
dc.date.available
2018-04-13T19:02:07Z
dc.date.issued
2005-04
dc.identifier.citation
Martinez, Maria Alejandra; Delgado, Osvaldo Daniel; Baigori, Mario Domingo; Siñeriz, Faustino; Sequence analysis, cloning and over-expression of an endoxylanase from the alkaliphilic Bacillus halodurans; Springer; Biotechnology Letters; 27; 8; 4-2005; 545-550
dc.identifier.issn
0141-5492
dc.identifier.uri
http://hdl.handle.net/11336/42032
dc.description.abstract
The BhMIR32 xyn11A gene, encoding an extracellular endoxylanase of potential interest in bio-bleaching applications, was amplified from Bacillus halodurans MIR32 genomic DNA. The protein encoded is an endo-1,4-β-xylanase belonging to family 11 of glycosyl hydrolases. Its nucleotide sequence was analysed and the mature peptide was subcloned into pET22b(+) expression vector. The enzyme was over-expressed in a high density Escherichia coli culture as a soluble and active protein, and purified in a single step by immobilised metal ion affinity chromatography with a specific activity of 3073 IU mg−1.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Bacillus Halodurans Mir32
dc.subject
Purification,
dc.subject
Over-Expression
dc.subject
Xylanase Sequence
dc.subject.classification
Otras Biotecnología Industrial
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Biotecnología Industrial
dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
dc.title
Sequence analysis, cloning and over-expression of an endoxylanase from the alkaliphilic Bacillus halodurans
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-04-10T14:07:27Z
dc.journal.volume
27
dc.journal.number
8
dc.journal.pagination
545-550
dc.journal.pais
Alemania
dc.journal.ciudad
Berlín
dc.description.fil
Fil: Martinez, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Lund University; Suecia
dc.description.fil
Fil: Delgado, Osvaldo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil
Fil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Lund University; Suecia
dc.description.fil
Fil: Siñeriz, Faustino. Lund University; Suecia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.journal.title
Biotechnology Letters
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1007/s10529-005-2879-2
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s10529-005-2879-2
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