Artículo

Improved method for separation of high molecular weight DNA molecules from Zymomonas mobilis

Estévez, Maria CristinaIcon ; Siñeriz, FaustinoIcon ; Abate, Carlos MauricioIcon
Fecha de publicación: 12/2000
Editorial: Springer
Revista: Biotechnology Letters
ISSN: 0141-5492
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:

Resumen

An improved method for separation of large DNA molecules from Zymomonas mobilis was developed using a contour-clampe homogeneous electric field technique. The preparation of DNA samples was optimized, as well as the migration conditions, to resolve the chromosomal DNA fractions obtained after digestion with SfiI. A 22s (45H) pulse setting gave maximal separation of 240 to 109 kb fragments range, while 9 s (40h), 3s (35h) and 1 s (30h) settings produced the best separation of 120 to 64 kb, 64 to 34 kb fragments range and less than 35 kb fragments, respectively.
Palabras clave: Migrtation Conditions , Zymomonas Mobilis
 
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Licencia
info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/41934
Colecciones
Articulos(PROIMI)
Articulos de PLANTA PILOTO DE PROC.IND.MICROBIOLOGICOS (I)
Citación
Estévez, Maria Cristina; Siñeriz, Faustino; Abate, Carlos Mauricio; Improved method for separation of high molecular weight DNA molecules from Zymomonas mobilis; Springer; Biotechnology Letters; 22; 16; 12-2000; 1273-1276
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