Show simple item record Hashim, Suhaila O. Delgado, Osvaldo Daniel Hatti-Kau,l Rajni Mulaa, Francis J. Mattiasson, Bo 2018-04-12T21:06:26Z 2004-05
dc.identifier.citation Hashim, Suhaila O.; Delgado, Osvaldo Daniel; Hatti-Kau,l Rajni; Mulaa, Francis J.; Mattiasson, Bo; Starch hydrolysing Bacillus halodurans isolates from a Kenyan soda lake; Springer; Biotechnology Letters; 26; 10; 5-2004; 823-828
dc.identifier.issn 0141-5492
dc.description.abstract Fourteen obligate alkaliphilic and halotolerant bacterial isolates, exhibiting extracellular amylase activity at 55thinsp°C and pH 10, were isolated from hot springs around Lake Bogoria, Kenya. From 16S rDNA sequence analysis, nine isolates shared 100% identity with Bacillus halodurans strain DSM 497T, while the rest shared 99% identity with alkaliphilic Bacillus species A-59. PCR of the intergenic spacer region between 16S and 23S rRNA genes (ISR-PCR) divided the isolates into two groups, while tDNA-PCR divided them into three groups. Bacillus halodurans DSM 497T had a different ISR pattern from the isolates, while it had a tDNA-PCR profile similar to the group that shared 99% identity with alkaliphilic Bacillus species A-59. All isolates hydrolysed soluble starch as well as amylose, amylopectin and pullulan. The amylase activity (1.2–1.8 U ml–1) in the culture broths had an optimum temperature of 55–65thinsp°C, was stimulated by 1 mm Ca2+, and was either partially (16–30%) or completely inhibited by 1 mm EDTA. Activity staining of the cell-free culture supernatant from the isolates revealed five alkaline active amylase bands.
dc.format application/pdf
dc.language.iso eng
dc.publisher Springer
dc.rights info:eu-repo/semantics/restrictedAccess
dc.subject.classification Biotecnología Medioambiental
dc.subject.classification Biotecnología del Medio Ambiente
dc.subject.classification INGENIERÍAS Y TECNOLOGÍAS
dc.title Starch hydrolysing Bacillus halodurans isolates from a Kenyan soda lake
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion 2018-04-10T13:53:54Z
dc.journal.volume 26
dc.journal.number 10
dc.journal.pagination 823-828
dc.journal.pais Alemania
dc.journal.ciudad Inglaterra
dc.description.fil Fil: Hashim, Suhaila O.. Lund University; Suecia
dc.description.fil Fil: Delgado, Osvaldo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil Fil: Hatti-Kau,l Rajni. Lund University; Suecia
dc.description.fil Fil: Mulaa, Francis J.. University of Nairobi; Kenia
dc.description.fil Fil: Mattiasson, Bo. Lund University; Suecia
dc.journal.title Biotechnology Letters
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/
dc.conicet.fuente individual

Archivos asociados

Blocked Acceso no disponible

This item appears in the following Collection(s)

Show simple item record

info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)