Artículo
Emulsan quantitation by Nile red quenching fluorescence assay
Fecha de publicación:
12/2004
Editorial:
Springer
Revista:
Applied Microbiology and Biotechnology
ISSN:
0175-7598
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
A Nile red fluorescent technique to quantify 20–200 μg ml−1 of emulsan was developed. Nile red dissolvedin DMSO showed an adsorption peak at 552 nm, and emission peak at 636 nm, with molar extinction coefficient of 19,600 cm−1 M−1. Nile red fluorescence in DMSO was proportionally quenched by emulsan and the quenching was time-dependent. The assay was used to follow the production of emulsan by cultures of Acinetobacter venetianus RAG-1.
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Articulos(PROIMI)
Articulos de PLANTA PILOTO DE PROC.IND.MICROBIOLOGICOS (I)
Articulos de PLANTA PILOTO DE PROC.IND.MICROBIOLOGICOS (I)
Citación
Castro, Guillermo Raul; Larson, Bridget K.; Panilaitis, Bruce; Kaplan, David L.; Emulsan quantitation by Nile red quenching fluorescence assay; Springer; Applied Microbiology and Biotechnology; 67; 6; 12-2004; 767-770
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