Artículo

Emulsan quantitation by Nile red quenching fluorescence assay

Castro, Guillermo RaulIcon ; Larson, Bridget K.; Panilaitis, Bruce; Kaplan, David L.
Fecha de publicación: 12/2004
Editorial: Springer
Revista: Applied Microbiology and Biotechnology
ISSN: 0175-7598
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:

Resumen

A Nile red fluorescent technique to quantify 20–200 μg ml−1 of emulsan was developed. Nile red dissolvedin DMSO showed an adsorption peak at 552 nm, and emission peak at 636 nm, with molar extinction coefficient of 19,600 cm−1 M−1. Nile red fluorescence in DMSO was proportionally quenched by emulsan and the quenching was time-dependent. The assay was used to follow the production of emulsan by cultures of Acinetobacter venetianus RAG-1.
 
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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/41927
DOI: http://dx.doi.org/10.1007/s00253-004-1849-2
URL: https://link.springer.com/article/10.1007/s00253-004-1849-2
Colecciones
Articulos(PROIMI)
Articulos de PLANTA PILOTO DE PROC.IND.MICROBIOLOGICOS (I)
Citación
Castro, Guillermo Raul; Larson, Bridget K.; Panilaitis, Bruce; Kaplan, David L.; Emulsan quantitation by Nile red quenching fluorescence assay; Springer; Applied Microbiology and Biotechnology; 67; 6; 12-2004; 767-770
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