Artículo
Isolation and characterization of indigenous copper-resistant actinomycete strains
Fecha de publicación:
09/2005
Editorial:
Elsevier Gmbh, Urban & Fischer Verlag
Revista:
Chemie Der Erde
ISSN:
0009-2819
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Fifty actinomycetes were isolated from copper contaminated and non-contaminated area. Primary qualitative screening assays showed that 100% of the isolated microorganisms of the contaminated area were resistant up to 80 mg L 1 of CuSO4. On the other hand, 100% of isolates from non-contaminated area grew at 16 mg L 1, 87.4% at 40 mg L 1 and only 19.4% of them were capable of growing at 80 mg L 1 of CuSO4. The semiquantitative assay showed that the isolated strains from the sediments of the contaminated site were resistant up to the highest concentration tested (1000 mg L 1) with the exception of AB2C strain; however, the strains isolated from non-contaminated sediments were sensitive to Cu2+ concentrations higher than 200 and 400 mg L 1, respectively. Microbial growth of AB0 strain in presence of 39 mg L 1 copper showed an inhibition of 32% after 6 days of incubation as compared to the control, and copper residual concentration indicated a reduction in the supernatant of 71.2% after 6 days of incubation: pellet acid digestion proved that copper was accumulated by the cells. 16S rDNA restriction digestion of 1300 bp amplicons with CfoI and HpaII showed only.
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Articulos(PROIMI)
Articulos de PLANTA PILOTO DE PROC.IND.MICROBIOLOGICOS (I)
Articulos de PLANTA PILOTO DE PROC.IND.MICROBIOLOGICOS (I)
Citación
Albarracín, Virginia Helena; Amoroso, Maria Julia del R.; Abate, Carlos Mauricio; Isolation and characterization of indigenous copper-resistant actinomycete strains; Elsevier Gmbh, Urban & Fischer Verlag; Chemie Der Erde; 65; 1; 9-2005; 145-156
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