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Artículo

Tagging the juvenile locus in soybean [Glycine max (L.) Merr.] with molecular markers

Cairo, Carlos Alberto; Stein, JulianaIcon ; Guelman, Sebastián A.; Delgado Benarroch, LucianaIcon ; Bortolotti, Santiago; Ortiz, Juan Pablo AmelioIcon ; Morandi, Eligio NatalioIcon
Fecha de publicación: 12/2002
Editorial: Springer
Revista: Euphytica
ISSN: 0014-2336
e-ISSN: 1573-5060
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Biológicas

Resumen

Soybean cultivars carrying the `long juvenile trait' show a delayed flowering response under short day conditions. The incorporation of this character into genotypes of agronomic interest may allow a broader range of sowing dates and latitudes for a single cultivar adaptation. The objective of this work was to identify molecular markers linked to the juvenile locus in soybean. Experiments were carried out using two pairs of near isogenic lines(NILs) differing in the presence of the long juvenile trait, and RAPD markers. Four hundred primers were first screened to find polymorphism associated with the trait. Additional differences between NILs were sought by digesting the genomic DNA with five restriction enzymes. Polymorphic fragments detected between NILs were tested for linkage to the juvenile locus in the corresponding F2 segregating populations. Marker bc357-HaeIII was linked (χ2L = 46.316) to the juvenile locus with an estimated recombination frequency of 0.13 ± 0.03in one of the genetic backgrounds studied. The fragment was cloned, sequenced and converted into a SCAR marker. Moreover,bc357-HaeIII was used as RFLP probe. Both, SCAR and RFLP generated markers linked to the juvenile locus in the two genetic backgrounds analysed. Results presented in this work can be utilised for both, the localisation of the gene associated with the character and for tagging the juvenile trait in soybean breeding programs.
Palabras clave: Glycine Max , Juvenile Locus , Molecular Markers , Rapd
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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/41818
DOI: http://dx.doi.org/10.1023/A:1015767623038
URL: https://link.springer.com/article/10.1023/A:1015767623038
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Articulos de INST.DE BOTANICA DEL NORDESTE (I)
Citación
Cairo, Carlos Alberto; Stein, Juliana; Guelman, Sebastián A.; Delgado Benarroch, Luciana; Bortolotti, Santiago; et al.; Tagging the juvenile locus in soybean [Glycine max (L.) Merr.] with molecular markers; Springer; Euphytica; 124; 3; 12-2002; 387-395
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