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Artículo

Homologous prime-boost strategy with TgPI-1 improves the immune response and protects highly susceptible mice against chronic Toxoplasma gondii infection

Sánchez, Vanesa RoxanaIcon ; Fenoy, Ignacio MartínIcon ; Picchio, Mariano SergioIcon ; Soto, Ariadna SoledadIcon ; Arcon, NadiaIcon ; Golgman, Alejandra; Martín, ValentinaIcon
Fecha de publicación: 07/2015
Editorial: Elsevier Science
Revista: Acta Tropica
ISSN: 0001-706X
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Parasitología

Resumen

Subunit-based vaccines are safer than live or attenuated pathogen vaccines, although they are generally weak immunogens. Thus, proper combination of immunization strategies and adjuvants are needed to increase their efficacy. We have previously protected C3H/HeN mice from Toxoplasma gondii infection by immunization with the serine protease inhibitor-1 (TgPI-1) in combination with alum. In this work, we explore an original vaccination protocol that combines administration of recombinant TgPI-1 by intradermal and intranasal routes in order to enhance protection in the highly susceptible C57BL/6 strain. Mice primed intradermally with rTgPI-1 plus alum and boosted intranasally with rTgPI-1 plus CpG-ODN elicited a strong specific Th1/Th2 humoral response, along with a mucosal immune response characterized by specific-IgA in intestinal lavages. A positive cellular response of mesentheric lymph node cells and Th1/Th2 cytokine secretion in the ileon were also detected. When immunized mice were challenged with the cystogenic Me49 T. gondii strain, they displayed up to 62% reduction in brain parasite burden. Moreover, adoptive transfer of mesenteric lymph node cells from vaccinated to naïve mice induced significant protection against infection. These results demonstrate that this strategy that combines the administration of TgPI-1 by two different routes, intradermal priming and intranasal boost, improves protective immunity against T. gondii chronic infection in highly susceptible mice.
Palabras clave: Mucosal Immunity , Prime-Boost Strategy , Serin Proteasa Inhibitor-1 , Subunit Vaccine , Toxoplasma Gondii
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/41766
DOI: http://dx.doi.org/10.1016/j.actatropica.2015.07.013
URL: https://www.sciencedirect.com/science/article/pii/S0001706X15300607
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Citación
Sánchez, Vanesa Roxana; Fenoy, Ignacio Martín; Picchio, Mariano Sergio; Soto, Ariadna Soledad; Arcon, Nadia; et al.; Homologous prime-boost strategy with TgPI-1 improves the immune response and protects highly susceptible mice against chronic Toxoplasma gondii infection; Elsevier Science; Acta Tropica; 150; 7-2015; 159-165
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