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dc.contributor.author
Arneodo Larochette, Joel Demián
dc.contributor.author
Balbi, Emilia I.
dc.contributor.author
Flores, Fernando M.
dc.contributor.author
Sciocco Cap, Alicia
dc.date.available
2018-04-11T19:10:10Z
dc.date.issued
2015-12
dc.identifier.citation
Arneodo Larochette, Joel Demián; Balbi, Emilia I.; Flores, Fernando M.; Sciocco Cap, Alicia; Molecular Identification of Helicoverpa armigera (Lepidoptera: Noctuidae: Heliothinae) in Argentina and Development of a Novel PCR-RFLP Method for its Rapid Differentiation From H. zea and H. gelotopoeon; Entomological Society of America; Journal of Economic Entomology; 108; 6; 12-2015; 2505-2510
dc.identifier.issn
0022-0493
dc.identifier.uri
http://hdl.handle.net/11336/41758
dc.description.abstract
Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae: Heliothinae) is among the most voracious global pests of agriculture. Adults of this species were identified recently in northern Argentina by dissection of male genitalia. In this work, a rapid and simple molecular tool was designed to distinguish H. armigera from the morphologically similar indigenous bollworms Helicoverpa zea (Boddie) and Helicoverpa gelotopoeon (Dyar), regardless of the life stage. Amplification of partial COI gene with a new primer pair, and subsequent digestion with endonuclease HinfI, yielded different RFLP profiles for the three main Helicoverpa pests currently present in South America. The method was validated in Helicoverpa specimens collected across Argentina, whose identity was further corroborated by COI sequencing and phylogenetic analysis. The data reported here constitute the first molecular confirmation of thispest in the country. The survey revealed the occurrence of H. armigera in northern and central Argentina, including the main soybean- and maize-producing area.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Entomological Society of America
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Old World Bollworm
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Helicoverpa
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South America
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Biological Invasion
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Barcode
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Otras Ciencias Biológicas
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Molecular Identification of Helicoverpa armigera (Lepidoptera: Noctuidae: Heliothinae) in Argentina and Development of a Novel PCR-RFLP Method for its Rapid Differentiation From H. zea and H. gelotopoeon
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-04-11T15:12:43Z
dc.journal.volume
108
dc.journal.number
6
dc.journal.pagination
2505-2510
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Lanham
dc.description.fil
Fil: Arneodo Larochette, Joel Demián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina
dc.description.fil
Fil: Balbi, Emilia I.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuaria Marcos Juárez; Argentina
dc.description.fil
Fil: Flores, Fernando M.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuaria Marcos Juárez; Argentina
dc.description.fil
Fil: Sciocco Cap, Alicia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina
dc.journal.title
Journal of Economic Entomology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1093/jee/tov254
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jee/article-abstract/108/6/2505/2379774
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