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dc.contributor.author
Naz, Nadia
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Dsouza, Roy N.
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Yelemane, Vikas
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Vennapusa, Rami Reddy
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Kangwa, Martin
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Fernandez Lahore, Hector
dc.date.available
2018-04-11T18:36:00Z
dc.date.issued
2015-07
dc.identifier.citation
Naz, Nadia; Dsouza, Roy N.; Yelemane, Vikas; Vennapusa, Rami Reddy; Kangwa, Martin; et al.; Growth-dependent surface characteristics of Hansenula Polymorpha: implications for expanded bed adsorption chromatography; Korean Soc Biotechnology & Bioengineering; Biotechnology And Bioprocess Engineering; 20; 3; 7-2015; 576-584
dc.identifier.issn
1226-8372
dc.identifier.uri
http://hdl.handle.net/11336/41749
dc.description.abstract
The cell surface characteristics of a methylotrophic wild-type strain of yeast, Hansenula polymorpha, was investigated at different growth stages (early log, late log, stationary and death) of the biomass under different conditions (low and high salt in intact and disrupted forms) using extended DLVO theory. Biomass was characterized by contact angle measurements as well as zeta potential determinations. These measurements were used to describe the hydrophobic, polar, and electrostatic behavior of the biomass in its growth stages. Consequently, interaction free energy vs. distance profiles of the biomass with anion-exchange and HIC adsorbents were conveniently generated. A strong interaction was calculated between cells and the adsorbents in the stationary and death phases of the biomass illustrated by the striking correlation between theoretical predictions and biomass deposition experiments. The physico-chemical properties of biomass in different growth phases have important implications for expanded bed adsorption chromatography, where unfavorable biomass-adsorbent interactions adversely affect process efficiency.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Korean Soc Biotechnology & Bioengineering
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Expanded Bed Adsorption
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Chromatography
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Extended Dlvo Theory
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Surface Energetics
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Cell Adhesion
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Hydrophobic Interaction
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Ion Exchange
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Microbial Growth
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Otras Ciencias Químicas
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Ciencias Químicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Growth-dependent surface characteristics of Hansenula Polymorpha: implications for expanded bed adsorption chromatography
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-04-11T15:14:03Z
dc.journal.volume
20
dc.journal.number
3
dc.journal.pagination
576-584
dc.journal.pais
Corea del Norte
dc.journal.ciudad
Seoul
dc.description.fil
Fil: Naz, Nadia. Jacobs University; Alemania
dc.description.fil
Fil: Dsouza, Roy N.. Jacobs University; Alemania
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Fil: Yelemane, Vikas. Jacobs University; Alemania
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Fil: Vennapusa, Rami Reddy. Shantha Biotechnics; India
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Fil: Kangwa, Martin. Jacobs University; Alemania
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Fil: Fernandez Lahore, Hector. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Jacobs University; Alemania
dc.journal.title
Biotechnology And Bioprocess Engineering
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s12257-014-0397-6
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs12257-014-0397-6
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