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Artículo

Citrus psorosis virus 24K protein interacts with citrus miRNA precursors, affects their processing and subsequent miRNA accumulation and target expression

Reyes Martinez, Carina AndreaIcon ; Ocolotobiche, Eliana EvelinaIcon ; Marmisollé, Facundo ErnestoIcon ; Robles Luna, GabrielIcon ; Borniego, María BelénIcon ; Bazinni, Ariel Alejandro; Asurmendi, SebastianIcon ; Garcia, Maria LauraIcon
Fecha de publicación: 05/2015
Editorial: Wiley Blackwell Publishing, Inc
Revista: Molecular Plant Pathology
ISSN: 1464-6722
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Biológicas

Resumen

Sweet orange (Citrus sinensis), one of the most important fruit crops worldwide, may suffer from disease symptoms induced by virus infections, thus resulting in dramatic economic losses. Here we show that the infection of sweet orange plants with two isolates of Citrus psorosis virus (CPsV) expressing different symptomatology alters the accumulation of a set of endogenous microRNAs (miRNAs). Within these miRNAs, miR156, miR167 and miR171 were the most downregulated with almost a 3?]fold reduction in the infected samples. This downregulation led to a concomitant upregulation of some of their targets, such as Squamosa promoter binding protein like 9 and 13 as well as Scarecrow?]like 6. The processing of miRNA precursors, pre?]miR156 and pre?]miR171, in sweet orange seems to be affected by the virus. For instance, the virus infection increases the level of unprocessed precursors, which is accompanied by the concomitant decrease of mature species accumulation. MiR156a primary transcript accumulation remained unaltered, thus strongly suggesting a processing deregulation for this transcript. The co?]immunoprecipitation of viral 24K protein with pre?]miR156a or pre?]miR171a suggests that the alteration in the processing of these precursors might be due to a direct or indirect interaction with this particular viral protein. This result is also consistent with the nuclear localization of both miRNA precursors and CPsV 24K protein. This study contributes to the understanding of the manner in which virus can alter host regulatory mechanisms, particularly miRNA biogenesis and target expression.
Palabras clave: Citrus Psorosis Virus , Mirnas , Symptons
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/41744
DOI: http://dx.doi.org/10.1111/mpp.12282
URL: https://onlinelibrary.wiley.com/doi/abs/10.1111/mpp.12282
Colecciones
Articulos(IBBM)
Articulos de INST.DE BIOTECNOLOGIA Y BIOLOGIA MOLECULAR
Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Citación
Reyes Martinez, Carina Andrea; Ocolotobiche, Eliana Evelina; Marmisollé, Facundo Ernesto; Robles Luna, Gabriel; Borniego, María Belén; et al.; Citrus psorosis virus 24K protein interacts with citrus miRNA precursors, affects their processing and subsequent miRNA accumulation and target expression; Wiley Blackwell Publishing, Inc; Molecular Plant Pathology; 17; 3; 5-2015; 317-329
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