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dc.contributor.author
Lucero Estrada, Cecilia Stella Marys
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Alcaráz, Lucia E.
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Satorres, Sara E.
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Manfredi, Eduardo
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Velázquez, Lidia del Carmen
dc.date.available
2018-04-10T20:25:26Z
dc.date.issued
2013-12
dc.identifier.citation
Lucero Estrada, Cecilia Stella Marys; Alcaráz, Lucia E.; Satorres, Sara E.; Manfredi, Eduardo; Velázquez, Lidia del Carmen; Presence of enterotoxigenic Staphylococcus aureus in artisan fruit salads in the city of San Luis, Argentina; Sociedade Brasileira de Microbiologia; Brazilian Journal of Microbiology; 44; 4; 12-2013; 1155-1161
dc.identifier.issn
1517-8382
dc.identifier.uri
http://hdl.handle.net/11336/41616
dc.description.abstract
An increase in the consumption of fruit juices and minimally processed fruits salads has been observed in recent years all over the world. In this work, the microbiological quality of artisan fruit salads was analysed. Faecal coliforms, Salmonella spp, Shigella spp, Yersinia enterocolitica and Escherichia coli O157:H7 were not detected; nevertheless, eleven strains of Staphylococcus aureus were isolated. By multiplex PCR, all isolates showed positive results for S. aureus 16S rRNA gene and 63.6% of them were positive for sea gene. Furthermore, PCR sea positive strains were able to produce the corresponding enterotoxin. Finally, the inactivation of these strains in fruit salads by nisin, lysozyme and EDTA, was studied. EDTA produced a total S. aureus growth inhibition after 60 h of incubation at a concentration of 250 mg/L. The presence of S. aureus might indicate inadequate hygiene conditions during salad elaboration; however, the enterotoxigenicity of the strains isolated in this study, highlights the risk of consumers intoxication. EDTA could be used to inhibit the growth of S. aureus in artisan fruit salads and extend the shelf life of these products.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Sociedade Brasileira de Microbiologia
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Staphylococcus Aureus,
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Enterotoxigenic Strains
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Fruit Salads
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Antimicrobial Compounds
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Multiplex Pcr
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Staphylococcus Aureus,
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Otras Ciencias Biológicas
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Presence of enterotoxigenic Staphylococcus aureus in artisan fruit salads in the city of San Luis, Argentina
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-04-10T19:03:10Z
dc.identifier.eissn
1678-4405
dc.journal.volume
44
dc.journal.number
4
dc.journal.pagination
1155-1161
dc.journal.pais
Brasil
dc.journal.ciudad
San Pablo
dc.description.fil
Fil: Lucero Estrada, Cecilia Stella Marys. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
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Fil: Alcaráz, Lucia E.. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
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Fil: Satorres, Sara E.. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
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Fil: Manfredi, Eduardo. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorio e Instituto de Salud ; Argentina
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Fil: Velázquez, Lidia del Carmen. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
dc.journal.title
Brazilian Journal of Microbiology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/mr9gxv
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3958181/
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info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1590/S1517-83822014005000001
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