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dc.contributor.author
Baron, Alessandra M.
dc.contributor.author
Sarquis, María Inés M.
dc.contributor.author
Baigori, Mario Domingo
dc.contributor.author
Mitchell, David A.
dc.contributor.author
Krieger, Nadia
dc.date.available
2018-04-10T17:02:48Z
dc.date.issued
2005-07
dc.identifier.citation
Baron, Alessandra M.; Sarquis, María Inés M.; Baigori, Mario Domingo; Mitchell, David A.; Krieger, Nadia; A comparative study of the synthesis of n-butyl-oleate using a crude lipolytic extract of Penicillum coryophilum in water-restricted environments; Elsevier Science; Journal of Molecular Catalysis B: Enzymatic; 34; 1-6; 7-2005; 25-32
dc.identifier.issn
1381-1177
dc.identifier.uri
http://hdl.handle.net/11336/41516
dc.description.abstract
We evaluated the potential of a lipolytic extract from Penicillium corylophilum for use in biocatalysis. Lipolytic activity was stable when the extract was incubated in the presence of hydrophobic solvents. In fact, when the extract was incubated in n-heptane with an initial water activity of 0.95, the activity increased by 30%. Synthesis of n-butyl-oleate in different systems was compared. In AOT/n-heptane reverse micelles, a 100% yield of ester was obtained within 12 h, with a water content (W0, [H2O]/[AOT]) of 10. Several macro-heterogeneous systems involving n-heptane as the solvent were also compared. Direct addition of lyophilised enzymatic preparation to this system gave a 100% yield in 48 h, with an initial water activity of 0.11. Performance was poorer with direct addition of enzymatic preparation co-lyophilised with β-cyclodextrin and with addition of enzymatic preparation immobilized on a hydrophobic gel, with yields significantly less than 100%. We conclude that the lipolytic extract of P. corylophilum can be used in synthesis reactions in low-water systems, which justifies further efforts to purify lipolytic enzymes from the extract and to undertake an in-depth evaluation of their potential for use in biocatalysis, for the production of fine chemicals.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Lipases
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Penicilium Corylophilum
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Biocatalysis
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Low-Water Systems
dc.subject.classification
Biología Celular, Microbiología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
A comparative study of the synthesis of n-butyl-oleate using a crude lipolytic extract of Penicillum coryophilum in water-restricted environments
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-04-10T13:53:25Z
dc.journal.volume
34
dc.journal.number
1-6
dc.journal.pagination
25-32
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Baron, Alessandra M.. Universidade Federal do Paraná; Brasil
dc.description.fil
Fil: Sarquis, María Inés M.. Colecâo de Cultura de Fungos; Brasil
dc.description.fil
Fil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil
Fil: Mitchell, David A.. Universidade Federal do Paraná; Brasil
dc.description.fil
Fil: Krieger, Nadia. Universidade Federal do Paraná; Brasil
dc.journal.title
Journal of Molecular Catalysis B: Enzymatic
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1016/j.molcatb.2005.04.002
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1381117705000433