Artículo
A Monoclonal Antibody Against the Extracellular Domain of Mouse and Human Epithelial V-like Antigen 1 Reveals a Restricted Expression Pattern Among CD4- CD8- Thymocytes
Garabatos, Nahir; Blanco, Jesus; Fandos, Cesar; Lopez, Elena; Santamaria, Pere; Ruiz, Andrea; Perez Vidakovics, Maria Laura Anabella
; Benveniste, Patricia; Galkin, Oleksandr; Zuñiga Pflucker, Juan Carlos; Serra, Pau
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Fecha de publicación:
10/2014
Editorial:
Mary Ann Liebert
Revista:
Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
ISSN:
2167-9436
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Expression of transcripts for the homotypic adhesion protein epithelial V-like antigen 1 (EVA1), also known as myelin protein zero like-2 (Mpzl2), is known to be present in thymic stromal cells. However, protein expression within different thymic subsets, stromal and/or lymphoid, has not been characterized due a lack of specific reagents. To address this, we generated a hybridoma (G9P3-1) secreting a monoclonal antibody (G9P3-1Mab), reactive against both human and mouse EVA1. The G9P3-1Mab was generated by immunizing Mpzl2-deficient gene-targeted mice with the extracellular domain of EVA1, followed by a conventional hybridoma fusion protocol, illustrating the feasibility of using gene-targeted mice to generate monoclonal antibodies with multiple species cross-reactivity. We confirmed expression of EVA1 on cortical and medullary epithelial cell subsets and revealed a restricted pattern of expression on CD4- CD8- double negative (DN) cell subsets, with the highest level of expression on DN3 (CD44lowCD25+) thymocytes. G9P3-1MAb is a valuable reagent to study thymic T cell development and is likely useful for the analysis of pathological conditions affecting thymopoiesis, such as thymic involution caused by stress or aging.
Palabras clave:
Eva1
,
Thymopoiesis
,
Double Negative Cell Subset
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Identificadores
Colecciones
Articulos(IBBM)
Articulos de INST.DE BIOTECNOLOGIA Y BIOLOGIA MOLECULAR
Articulos de INST.DE BIOTECNOLOGIA Y BIOLOGIA MOLECULAR
Citación
Garabatos, Nahir; Blanco, Jesus; Fandos, Cesar; Lopez, Elena; Santamaria, Pere; et al.; A Monoclonal Antibody Against the Extracellular Domain of Mouse and Human Epithelial V-like Antigen 1 Reveals a Restricted Expression Pattern Among CD4- CD8- Thymocytes; Mary Ann Liebert; Monoclonal Antibodies in Immunodiagnosis and Immunotherapy; 33; 5; 10-2014; 305-311
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