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dc.contributor.author Soria, Ivana
dc.contributor.author Quattrocchi, Valeria
dc.contributor.author Langellotti, Cecilia Ana
dc.contributor.author Gammella, Mariela
dc.contributor.author Digiacomo, Sebastian
dc.contributor.author Garcia de la Torre, Beatriz
dc.contributor.author Andreu, David
dc.contributor.author Montoya, Maria
dc.contributor.author Sobrino, Francisco
dc.contributor.author Blanco, Esther
dc.contributor.author Zamorano, Patricia Ines
dc.date.available 2018-04-06T17:53:15Z
dc.date.issued 2017-09
dc.identifier.citation Soria, Ivana; Quattrocchi, Valeria; Langellotti, Cecilia Ana; Gammella, Mariela; Digiacomo, Sebastian; et al.; Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle; Public Library of Science; Plos One; 12; 9; 9-2017; 1-16; e0185184
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/11336/41182
dc.description.abstract Foot-and-mouth disease virus (FMDV) causes a highly contagious disease in cloven-hoofed animals. A synthetic vaccine candidate consisting of dendrimeric peptides harbouring two copies of a B-epitope [VP1(136–154)] linked to a T-cell epitope [3A(21–35)] of FMDV confers protection to type O FMDV challenge in pigs. Herein we show in cattle that novel dendrimeric peptides bearing a T-cell epitope [VP1(21–40] and two or four copies of a B-cell epitope [VP1(135–160)] from type O1 Campos FMDV (termed B2T and B4T, respectively) elicited FMDV specific immune responses to similar levels to a commercial vaccine. Animals were challenged with FMDV and 100% of vaccinated cattle with B2T or B4T were protected to podal generalization. Moreover, bovines immunized with B4T were completely protected (with no clinical signs) against FMDV challenge after three vaccine doses, which was associated with titers of viral neutralizing antibodies in serum higher than those of B2T group (p< 0.05) and levels of opsonic antibodies similar to those of animals immunized with one dose of FMDV commercial vaccine. Bovines vaccinated with both dendrimeric peptides presented high levels of IgG1 anti FMDV in sera and in mucosa. When IgA in nasal secretions was measured, 20% or 40% of the animals in B2T or B4T groups respectively, showed anti-FMDV IgA titers. In addition, B2T and B4T peptides evoked similar consistent T cell responses, being recognized in vitro by lymphocytes from most of the immunized cattle in the proliferation assay, and from all animals in the IFN-γ production assay. Taken together, these results support the potential of dendrimers B2T or B4T in cattle as a highly valuable, cost-effective FMDV candidate vaccine with DIVA potential.
dc.format application/pdf
dc.language.iso eng
dc.publisher Public Library of Science
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject DENDRIMERIC PEPTIDES
dc.subject PROTECTION
dc.subject CATTLE
dc.subject FOOT-AND-MOUTH DISEASE
dc.subject.classification Otras Ciencias Biológicas
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2018-04-06T13:44:35Z
dc.journal.volume 12
dc.journal.number 9
dc.journal.pagination 1-16; e0185184
dc.journal.pais Estados Unidos
dc.journal.ciudad San Francisco
dc.description.fil Fil: Soria, Ivana. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Quattrocchi, Valeria. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Langellotti, Cecilia Ana. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Gammella, Mariela. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
dc.description.fil Fil: Digiacomo, Sebastian. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
dc.description.fil Fil: Garcia de la Torre, Beatriz. Universitat Pompeu Fabra; España
dc.description.fil Fil: Andreu, David. Universitat Pompeu Fabra; España
dc.description.fil Fil: Montoya, Maria. The Pirbright Institute; Reino Unido
dc.description.fil Fil: Sobrino, Francisco. Centro de Biología Molecular “Severo Ochoa”; España
dc.description.fil Fil: Blanco, Esther. Centro de Investigación en Sanidad Animal; España
dc.description.fil Fil: Zamorano, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
dc.journal.title Plos One
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1371/journal.pone.0185184
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0185184
dc.conicet.fuente Elsevier


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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)